实用医学杂志2017，Vol.33Issue(6)：867-871,5.DOI:10.3969/j.issn.1006-5725.2017.06.005
miR-25靶向抑制KLF4促进胃癌细胞增殖
miR-25 promotes gastric cancer cells proliferation by directly targeting KLF4
丁小丽 1夏宇 1胡蓉2
作者信息
- 1. 341000 江西省赣州市,赣南医学院
- 2. 341000 江西省赣州市,赣南医学院第一附属医院
- 折叠
摘要
Abstract
ObjectiveTo investigate whether miR-25 targets KLF4 to regulate the growth of gastric cancer cells and lucidate the mechanism of miR-25 producing functional effects in gastric cancer cells. Methods The expression levels of miR-25 and KLF4 were detected by qRT-PCR in 35 pairs of gastric cancer tissues of patients. Target genes of miR-25 were predicted using Targetscan and verified though dual luciferase activity assay and west-ern blot. MTT assay and clone formation assay were detected after downregulated miR-25 and overexpressed KLF4 respectively. Results miR-25 was highly expressed in gastric cancer tissues with KLF4 downexpresstion. Downreg-ulated miR-25 and overexpressed KLF4 respectively can significantly reduce the proliferation of gastric cancer cells,the results were opposite when overexpression of KLF4. Conclusion miR-25 promotes gastric cancer cells proliferation by targeting KLF4.关键词
胃肿瘤/miR-25/KLF4/ERK通路Key words
Stomach neoplasms miR-25/KLF4/ERK Pathway引用本文复制引用
丁小丽,夏宇,胡蓉..miR-25靶向抑制KLF4促进胃癌细胞增殖[J].实用医学杂志,2017,33(6):867-871,5.
