现代妇产科进展2017，Vol.26Issue(4)：246-250,5.DOI:10.13283/j.cnki.xdfckjz.2017.04.002
MiR-204降调Caveolin-1表达降低HRGECs对大分子蛋白的通透性
miR-204 decrease the permeability of human glomerular endothelial cells to macromolecular protein through down-regulating of caveolin-1
摘要
Abstract
Objective:To explore the effects of microRNA-204 on caveolin-1 expression and permeability of human glomerular endothelial cells(HRGECs).Methods:The putative miR-204 targeted on CAV1 were predicted by means of the bioinformatics methods.HRGECs were cultured in vitro and 2 groups were devided.Control group was treated with the serum of normal pregnant and PE group was treated with the preeclampsia patients serum.Then we transfected HRGECs with exogenous miRNAs.HRGECs were separately transfected with miR-204 mimics,miR-204 inhibitor,miR-204 mimic NC sequence and miR-204 inhibitor NC sequence and cultured for 48 hours.The expressions of miR-204 and CAV1 mRNA were detected by qPCR.Western blot was used to detect the quantity of caveolin-1 expression.The permeability of HRGECs was tested by Evans-blue conjuncted BSA in transwell chambers.Result:QPCR results showed that the expression of miR-204 displayed a marked decrease after treated with PE serum while increase after transfected with miR-204 mimics in HRGECs and in miR-204 down-regulated group,the expression of miR-204 was reduced (P<0.05).The caveolin-1 expression was significantly inhibited when treated with miR-204 mimics and was enhanced in miR-204 inhibited group (P<0.05).The expression of CAV1 mRNA had no noted differences in both group.MiR-204 inhibited the permeability of HRGECs more obvious in miR-204 overexpressed group than that in control groups(P<0.05).Conclusion:MiR-204 may regulate the expression of caveolin-1 and the permeability of HRGECs by inhibiting the process of translation of CAV1.MiR-204 may participate in proteinuria formation in preeclampsia patients.关键词
miR-204/Caveolin-1/肾小球内皮细胞/屏障障碍/蛋白尿Key words
miR-204/Caveolin-1/HRGEC/Barrier dysfunction/Proteinuria分类
医药卫生引用本文复制引用
杜菲,徐钦洋,蒋荣珍..MiR-204降调Caveolin-1表达降低HRGECs对大分子蛋白的通透性[J].现代妇产科进展,2017,26(4):246-250,5.基金项目
国家自然科学基金资助项目(No:81570444) （No:81570444）
国家科技部"十二五"支撑计划项目(No:2014BAI05B05) （No:2014BAI05B05）
