现代妇产科进展2017，Vol.26Issue(4)：251-254,4.DOI:10.13283/j.cnki.xdfckjz.2017.04.003
低葡萄糖高乳酸环境对Hela细胞生存的影响
The effect of low glucose and high lactic acid on the survival of Hela
摘要
Abstract
Objective:To explore the effect of the low glucose and high lactic acid environment of survival of cervical carcinoma cells,and the regulation of EGFR-mTOR signal way.Methods:Hela cells were cultured in four conditions:normal glucose (glucose 10mmol/L),low glucose (glucose 3mmol/L),high lactic acid (lactic acid 2.5mmol/L) and low glucose add high lactic acid (glucose 3mmol/L,lactic acid 2.5mmol/L).Growth inhibition rate of Hela cell was determined by CCK-8.Flow cytometry (FCM)were performed to evaluate the cell cycle.The expression levels of EGFR and mTOR mRNA were detected using real-time quantitative polymerase chain reaction (real-time PCR).Results:Compared with those in regular sugar environment,the cell growth inhibition rates were significantly increased in high lactic acid environment(P<0.01).At 48h,the rate of G1/G0 was significantly increased (P<0.01),while the apoptosis rate of the cells and the expressions of EGFR and mTOR mRNA had no change.Compared with those in regular sugar environment,the cell growth inhibition rates were significantly increased in high lactic acid environment,(P<0.01),while lower than those in low glucose environment(P<0.01) and in high acid environment(P<0.05).The rates of G1,G2 and S phase had no change.The expressions of EGFR mRNA was reduced (P<0.05).The cell growth inhibition rates were significantly increased in high lactic acid add low glucose environment (P<0.01).At 48h,the apoptosis rate and the rate of G1/G0 were significantly increased (P<0.01),while the EGFR and mTOR expression levels were also increased (P<0.05).Conclusion:Hela in the low glucose add high lactic acid survives better than those in low glucose and in high lactic acid environment,while the expression of EGFR and mTOR is increased.关键词
宫颈癌/葡萄糖/乳酸/表皮生长因子受体/雷帕霉素受体Key words
Cervical Carcinoma/Glucose/Lactic Acid/EGFR/mTOR分类
医药卫生引用本文复制引用
李苹,孙蓬明,李丽娜,李明珠,吕育纯,林丽蓉..低葡萄糖高乳酸环境对Hela细胞生存的影响[J].现代妇产科进展,2017,26(4):251-254,4.基金项目
泉州市科技计划立项项目(No:2015Z41) （No:2015Z41）
国家面上项目(No:81672094) （No:81672094）
