现代妇产科进展2017，Vol.26Issue(4)：255-257,3.DOI:10.13283/j.cnki.xdfckjz.2017.04.004
二甲双胍对卵巢癌细胞侵袭迁移及MTA1表达的影响
Effect of metformin on the invasion and migration of ovarian cancer cells and the expression of MTA
邹歌 1董建新 2陈燕 2韩翠娜 1李丹丹 1唐雅娟2
作者信息
- 1. 华北理工大学研究生院,唐山 063000
- 2. 华北理工大学附属医院妇产科,唐山 063000
- 折叠
摘要
Abstract
Objective:To investigate the influence of metformin regulation on SKOV3 in migration and invasion of ovarian cancer.Methods:The human ovarian cancer SKOV3 cells were treated with metformin at a dose of 0,2.5,5.0 and 10.0mmol/L,cell migration and invasion were detected by cell scratch test and Transwell chamber assay respectively.The protein expression level of MTA1was detected by Western blotting,The mRNA expression of MTA1 was evaluated by real-time quantitative PCR.Results:Compared with the control group (0mmol/L),the cell migration rate of metformin intervention group decreased significantly (P<0.01),and no difference was found between 2.5mmol/L and 5mmol/L after 24h(P>0.05).The number of the cells which invaded matrigel was in a dose-dependent manner.Real-time quantitative PCR showed that the mRNA expression level of MTA1 was decreased in metformin treatment groups,significantly different from those in the control group (P<0.05).Western blot showed that the protein level of MTA1 was significantly decreased in metformin treatment groups (2.5,5.0 and 10.0mmol/L) when compared with the control group (P<0.05).Conclusions:Metformin inhibit invasion and migration of human ovarian cancer cells,this inhibition was in a dose-dependent manner,and may be related to the inhibition of MTA1 expression.关键词
卵巢癌/二甲双胍/MTA1/侵袭/迁移Key words
Ovarian Cancer/Metformin/MTA1/Invasion/Migration分类
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邹歌,董建新,陈燕,韩翠娜,李丹丹,唐雅娟..二甲双胍对卵巢癌细胞侵袭迁移及MTA1表达的影响[J].现代妇产科进展,2017,26(4):255-257,3.
