新疆农业科学2017，Vol.54Issue(1)：60-65,6.DOI:10.6048/j.issn.1001-4330.2017.01.008
库尔勒香梨成熟果实石细胞含量与POD4基因表达量的相关性分析
Correlation Analysis of Solid Stone Cell Content and Expression of POD4 Gene in Korla Fragrant Pear
摘要
Abstract
[Objective] To study the correlation of stone cell content and POD4 gene expression in rough skin pear and normal skin fruit of mature Korla Fragrant Pear in the hope of providing molecular basis for improving the fruit quality.[Method] Rough skin fruit and normal skin fruit of Korla Fragrant Pear during the mature period were used as materials,and the firmness and the content of stone cell were determinated.Total RNA was extracted from fragrant pear fruit pulp,quantification expression of POD4 gene was determinated by Real-time fluorescent quantitative polymerase chain reaction (qRT PCR),the correlation of the firmness and the content of stone cell and quantification expression of POD4 gene were analyzed.[Result] The content of fruit stone cell was between 0.4 and 0.52 gram,pear firmness was between 245.22 and 314.71 PA and quantification expression of POD4 gene in mature pear was between 106.89 and 743.29.[Conclusion] The content of stone cell in rough skin fruit is 1.3 times than the normal skin fruit,the hardness was 1.28 times,POD4 gene expression was 6.59 times.The correlation between the content of stone cell and the quantification expression of POD4 gene was significantly positive (r =0.823),and correlation between the content of stone cell and the fruit hardness was positive (r =0.783).The correlation between quantification expression of POD4 and fruit firmness was positive (r =0.770).Therefore,the level of expression of POD gene can be used as a reference index for the identification of the content of stone cell.关键词
库尔勒香梨/石细胞含量/qRT-PCRKey words
Korla Fragrant Pear/content of stone cells/qRT-PCR分类
农业科技引用本文复制引用
李文慧,冯建荣,唐章虎,董胜利,徐麟,章世奎,张龑..库尔勒香梨成熟果实石细胞含量与POD4基因表达量的相关性分析[J].新疆农业科学,2017,54(1):60-65,6.基金项目
Supported by the germplasm resources preservation project of the Ministry of Agriculture (2016NWB019)农业部种质资源保种项目 (2016NWB019) （2016NWB019）
