新疆农业科学2017，Vol.54Issue(1)：132-139,8.DOI:10.6048/j.issn.1001-4330.2017.01.017
高放射土壤中拮抗放线菌菌株的筛选及鉴定
The Screening and Identification of Antagonistic Actinomycetes Strains from the High Radiation Soil
摘要
Abstract
[Objective] This project aims to isolate and screen 70 actinomycetes strains from the high radiation soil of Xinjiang by antagonistic effect of 8 kinds of pathogenic bacteria such as the Valsamali Miyabe et Yamada.[Method] By the plate confrontation culture method,and through initial screening and repeated screening of antagonistic effect of 8 kinds of pathogenic bacteria,and according to the morphological characteristics of the strains of CH10,the physiological and biochemical characteristics and 16S rDNA sequence went through identification and analysis.[Result] The results showed that 8 kinds of important pathogens of Xinjiang crops,such as the Valsa mali Miyabe et Yamada,were screened out from 70 kinds of actinomycetes,which had good antagonistic effect called CH10.[Conclusion] Strain CH10 belongs to the genus streptomyces and it has good antagonistic effect on the Valsa mali Miyabe et Yamada,which has provided a foundation for the biological control of the disease.关键词
拮抗放线菌/病原菌/苹果腐烂病/16S rDNA/链霉菌Key words
antagonistic actinomycetes/pathogenic bacteria/16S rDNA identification/streptomyces分类
农业科技引用本文复制引用
李阳,李健强,宋素琴,王静,楚敏..高放射土壤中拮抗放线菌菌株的筛选及鉴定[J].新疆农业科学,2017,54(1):132-139,8.基金项目
Supported by Key Projects in the National Science & Technology Pillar Program during the Twelfth Five-year Plan Period.(2012BAD42B02) （2012BAD42B02）
The project of the introduction of the higher-grade talented persons of the Xinjiang Uygur Autonomous Region(2015-2017)新疆科技援疆项目(201491150) （2015-2017）
高层次引进人才(2015-2017) （2015-2017）
