畜牧与兽医2017，Vol.49Issue(4)：24-28,5.
关岭牛FoxO6基因原核表达载体的构建及生物信息学分析
Bioinformotion analysis of Guanling cattle FoxO6 gene and construction of its prokaryotic expression vector
摘要
Abstract
The experiment aimed to analysis functions of the FoxO6 gene.We obtained the CDS region of Guarding cattle FoxO6 gene by the method of PCR and then constructed the recombination plasmid pET32a-FoxO6 and analyzed the sequence of FoxO6 protein successfully.The CDS region of Guanling cattle FoxO6 gene had 942 bp,encodes 313 amino acids and the protein weights 33.60 kD.The protein was hydrophilic,the isoelectric point of FoxO6 protein was about 9.44 and had positive charges in vivo.The protein had mainly random coil and α-helix concerning the secondary structure prediction,no signal peptide and the transmembrane helical structure showing an intracellular protein,mainly existed in nucleus.The recombination expression protein of FoxO6 had His tag etc,encoded 492 amino acids and weights 53.0 ku.The experiment preliminary analyzed physic-chemical properties and structure characteristics providing the basis theoretical knowledge for studying function and mechanism of the FoxO6 protein in vivo .关键词
关岭牛/FoxO6基因/原核表达载体/生物信息学分析Key words
Guanling cattle/FoxO6 gene/prokaryotic expression vector/bioinformotion analysis分类
生物科学引用本文复制引用
赵焕平,许厚强,陈伟,周迪,孙成娟,张青青,杨洋..关岭牛FoxO6基因原核表达载体的构建及生物信息学分析[J].畜牧与兽医,2017,49(4):24-28,5.基金项目
国家自然科学基金(31571279) （31571279）
促进与美大地区科研合作与高层次人才培养项目(教外司美[2016] 1481号) （教外司美[2016] 1481号）
