畜牧与兽医2017，Vol.49Issue(4)：74-78,5.
弓形虫致密颗粒蛋白GRA12的生物信息学分析
Bioinformatics analysis of dense granule protein GRA12 of Toxoplasma gondii
摘要
Abstract
In order to explore the possibility as a vaccine candidate antigen of the dense granule protein 12 (GRA12) of Toxoplasma gondii,the GRA12 gene sequence was obtained from the GenBank database,and then an online expert system for protein analysis was utilized to analyze the coding region of the gene sequence in combination with other bioinformatics softwares including GENE Runner,DNAMAN,DNASTAR,and so on.The physical and chemical properties,signal peptide,transmembrane domain,secondary structure,hydrophilicity /hydrophobicity and antigen epitopes of the gene encoding protein were predicted.The results showed that the gene was 1311 bp in full length.It encodes a protein of 436 amino acids.The encoded protein has stable physical and chemical properties,and has a theoretical molecular mass of 47.8 ku without signal peptides.The GRA12 protein has 6 transmembrane regions,14 serine phosphorylation sites,7 threonine phosphorylation sites,3 tyrosine phosphorylation sites,18 O-glycosylation sites,3 acetylated protein attachment sites,1 tyrosine sulfation sites,1 potential N-glycosylation sites,1 palmitoylation sites and 15 potential antigen epitopes.The analysis provides the theoretical basis for GRA12 as a protective antigen of T.gondii.关键词
弓形虫/GRA12/生物信息学/蛋白质结构/抗原表位Key words
Toxoplasma gondii/dense granule protein 12/bioinformatics/protein structure/antigenic epitopes分类
生物科学引用本文复制引用
王英贺,曹利利,郭衍冰,董航,宫鹏涛,苑淑贤,姚新华,魏峰..弓形虫致密颗粒蛋白GRA12的生物信息学分析[J].畜牧与兽医,2017,49(4):74-78,5.基金项目
国家自然科学基金青年基金项目(31302076) （31302076）
吉林省留学人员科技创新创业项目(2012) （2012）
吉林省公益性科研单位基本科研专项社会公益研究(2060302) （2060302）
国家自然科学基金(31472183) （31472183）
