畜牧与兽医2017，Vol.49Issue(4)：91-94,4.
蛇床子素体外抗鸽毛滴虫的效果及形态学观察
Observation on in vitro trichomonacidal effect of osthole and morphological changes of Trichomonas gallinae
摘要
Abstract
In order to investigate the trichomonacidal effect of osthol and morphological change of Trichomonas gallinae,four groups with different concentrations of osthole were set.At 6,8,12 and 24 h after culture,samples were taken for observation of the morphological changes of T.gallinae by optical and the electron microscopes,and the number of death was recorded.The results showed that with the increase of osthole concentration,T.gallinae mortality was increased,and the time of death was shortened.The 5 mg/mL group had the best efficacy since 12 h treatment could kill all Trichomonas;in lmg/mL group,no death was found until 24 h;the concentration of 3 mg/mL could lead all to death.The observation by scanning electron microscopy showed that at the initial stage of drug effect,T.gallinae gradually turned round from pear shape,and formed a complete body.Mter that,blurry edges,surface wrinkles and local sag could be observed.Then sag area increased,internal voids formed,body shape became irregular,and finally the worm was collapsed into pieces.The results indicated that osthole has obvious treatment effect against T.gallinae.关键词
蛇床子素/鸽毛滴虫/杀虫效应/形态学Key words
osthole/Trichomonas gallinae/trichomonacidal effect/morphology分类
农业科技引用本文复制引用
陈海华,薛勇,金李萍,沙奕羽,戴鼎震,刘家国..蛇床子素体外抗鸽毛滴虫的效果及形态学观察[J].畜牧与兽医,2017,49(4):91-94,4.基金项目
南京市生物农业项目资助(编号2013-32) （编号2013-32）
南京市科技计划项目资助(编号201201026) （编号201201026）
