眼科新进展2017，Vol.37Issue(4)：313-316,4.DOI:10.13389/j.cnki.rao.2017.0079
秋水仙碱对人眼Tenon囊成纤维细胞增殖及凋亡的影响
Effects of colchicine on proliferation and apoptosis of human Tenon's capsule fibroblasts
摘要
Abstract
Objective To investigate the effects of colchicine on the proliferation and apoptosis of human Tenon's capsule fibroblasts (HTCFs) in vitro.Methods HTCFs were cultured in vitro,and the subcultured cells were identified.MTS assay was used to observe the inhibitory actions of colchicine on HTCFs at different concentrations.The apoptotic rates of the cells were detected by flow cytometry.The apoptosisrelated proteins,including PARP,Caspase-9,Caspase-3 and active-Caspase-3,were detected by Western blot.Results MTS assay showed that colchicine inhibited the proliferation of HTCFs in a dose-and time-dependent manner.Flow cytometry showed that colchicine induced a dose-dependent apoptosis of HTCFs for 48 hours,the apoptotic rates of 5 μmol · L-1,10 μmol · L-1 and 20 μmol · L-1 were (18.37 ±4.26)％,(33.80 ±5.02)％ and (52.00 ± 2.00)％,respectively,there were significant differences compared with the control (F =91.59,P ＜ 0.001).Western blot showed the activation of Caspase-3 and PARP followed by colchicine treatment.Conclusion Colchicine significantly inhibits the proliferation of HTCFs in vitro,and induced apoptosis,which may be associated with the activation of mitochondrial apoptotic pathways.关键词
秋水仙碱/Tenon囊成纤维细胞/青光眼/增殖/凋亡/流式细胞术Key words
colchicine/Tenon's capsule fibroblasts/glaucoma/proliferation/apoptosis/flow cytometry分类
医药卫生引用本文复制引用
孔蕾,胡敏,华启云,张荭..秋水仙碱对人眼Tenon囊成纤维细胞增殖及凋亡的影响[J].眼科新进展,2017,37(4):313-316,4.基金项目
国家自然科学基金资助(编号:81560168)National Natural Science Foundation of China (No:81560168) （编号:81560168）
