眼科新进展2017，Vol.37Issue(4)：317-320,4.DOI:10.13389/j.cnki.rao.2017.0080
强光照致TgAPPswePS1转基因鼠视网膜功能障碍和结构损伤
Light exposure resulted in APPswe/PS1 transgenic mice retinal structural and functional disorder
摘要
Abstract
Objective To research the functional and structural change in TgAP-PswePS1 transgenic mice after intensive light exposure insult.Methods APPswe/PS1 transgenic mice at the age of 6 months old were grouped for experiments,the transgenic mice were replaced by light insult device for 6 months,while the control mice were kept in normal conditions.After 6 months light exposure,the eyes of control and experimental mice were examined with electroretinography (ERG).The retinal morphology change was investigated with H&E staining.All of the results were quantified and statistically analyzed.Results In the control group,the amplitudes of a and b wave in the rod response were (18.33 ±3.53) μV and (107.58 ± 14.72) μV,while (64.80 ±7.57) μV and (178.76 ± 14.47) μV for the amplitudes of a and b wave in the maximum response;After treated by 6 months of intensive light exposure,in experimental group mice,the amplitudes of a and b wave in the rod response were (17.92 ±4.89) μV and (21.83 ± 5.51) μV;While in the maximum response a striking decrease was detected with a wave (18.23 ±4.44) μV and b wave (24.50 ± 4.49) μV,by compared with control group,the difference were statistical significant (all P ＜ 0.05).Histopathological analysis found significant loss of outer nuclear layer,photoreceptor out segment,whereas controls remained little change in the retina.And the retinal thickness decreased significantly from (181.32 ± 13.47) μm in control group to (102.34 ±9.38) μm after light insults in experimental group,the difference was statistical significant (P =0.017).Conclusion Intensive light exposure can cause the retinal structural and functional disorder in the AP-Pswe/PS1 transgenic mouse.关键词
光损伤/视网膜电生理/转基因鼠/阿尔茨海默病Key words
light insult/electroretinography/transgenic mouse/Alzheimer's disease分类
医药卫生引用本文复制引用
董志章,李娟,孙雪荣,葛坚..强光照致TgAPPswePS1转基因鼠视网膜功能障碍和结构损伤[J].眼科新进展,2017,37(4):317-320,4.基金项目
国家自然科学基金资助(编号:81400424) （编号:81400424）
陕西省科学技术研究发展计划项目(编号:2014K11-03-07-04) （编号:2014K11-03-07-04）
温州市科技计划项目(编号:Y20140140)National Natural Science Foundation of China (No:81400424) （编号:Y20140140）
Science and Technology Research and Development Project of Shaanxi Province (No:2014K11-03-07-04) （No:2014K11-03-07-04）
Science and Technology Research and Development Project of Wenzhou (No:Y20140140) （No:Y20140140）
