湖北医药学院学报2016，Vol.35Issue(4)：333-337,341,6.DOI:10.13819/j.issn.1006-9674.2016.04.001
CIP2A在三阴性乳腺癌中的表达及作用机制研究
Research on the Expression of CIP2A in Human Triple Negative Breast Cancer and Its Mechanism
摘要
Abstract
Objective To investigate the biological function of cancerous inhibitor of protein phosphatase 2A(CIP2A) in human triple negative breast cancer (TNBC).Methocds The expression of CIP2A in TNBC cells was determined by Western blot analysis and real-time quantitative PCR(QPCR);siRNA was used for transfecting TNBC cells and then to detect the variation of invasiveness in the cells being transfected.The expression of Akt/mTOR/P70S6K pathway proteins was determined by Western blot.Results The expression of CIP2A in TNBC cell line was higher than that in low invasive breast cancer cells.After knocking out the CIP2A gene,the proliferation,invasion and metastasis of TNB cells were inhibited but the apoptosis and autophagy were induced.Meanwhile,the activation of Akt/mTOR/P70S6K signal pathway was inhibited.Conclusion These results validated the role of CIP2A as an invasion relevant oncoprotein and establish CIP2A as a promis ing therapeutic target of TNBC.关键词
CIP2A/三阴性乳腺癌/增殖/侵袭/自噬Key words
CIP2A/Triple negative breast cancer/Proliferation/Invasion/Autophagy引用本文复制引用
张亮,黄秋月,王配军,肖香玲,段超,范春生,刘莹..CIP2A在三阴性乳腺癌中的表达及作用机制研究[J].湖北医药学院学报,2016,35(4):333-337,341,6.基金项目
2014年度湖北医药学院优秀中青年科技创新团队资助计划项目(2014CXX05) （2014CXX05）
湖北医药学院2015年度研究生启动基金资助计划项目(2015QDJZR16) （2015QDJZR16）
2016年大学生创新创业训练计划项目(201610929001) （201610929001）
