湖北医药学院学报2016，Vol.35Issue(4)：342-344,349,4.DOI:10.13819/j.issn.1006-9674.2016.04.003
甲基化转移酶抑制剂zebularine对人子宫内膜癌细胞ECC-1高迁移率族蛋白B1基因表达的影响
The Effects of Zebularine on HMGB1 mRNA Expression in ECC-1 Cell Line
董毅飞 1王旭 1刁红录 1王伟明 1张昌军 1王华1
作者信息
- 1. 湖北医药学院附属人民医院生殖医学中心,湖北十堰442000
- 折叠
摘要
Abstract
Objective To study the effects of DNA methylation inhibitor zebularine on the proliferation,migration and high mobility group box-1 (HMGB1) mRNA expression in ECC-1 cell line(human endometrial carcinoma cell line).Methods MTT method and wound healing assay were used to evaluate the proliferation and migration level of ECC-1.The mRNA expression of HMGB1 was evaluated by Real-time PCR.Results After treatment with zebularine at a final concentration of 200 μmol/L for 24 h,the proliferation and migration of ECC-1 cell lines were significantly inhibited,the absorbance value of MTT method was 0.46±0.04 in theexperiment group,which was significantly lower than that in the control group(0.64± 0.05),(P＜0.05);the wound healing rate was 45.23％±3.45％ in the experiment group,which was significantly lower than that in the control group(87.45％±6.05％),(P＜0.05);the relative mRNA expression of HMGB1 was 1.02±0.10 in control group,and which was significantly higher than that in experiment group(0.25±0.06),(P＜0.05).Conclusion DNA methylation inhibitor zebularine could effectively inhibit the proliferation and migration of ECC-1 cell lines,and the effects may re late with the decreasing of HMGB1 mRNA expression.关键词
甲基化转移酶抑制剂/子宫内膜癌/高迁移率族蛋白Key words
Methylation transferase inhibitor/Endometrial carcinoma/High mobility group box-1引用本文复制引用
董毅飞,王旭,刁红录,王伟明,张昌军,王华..甲基化转移酶抑制剂zebularine对人子宫内膜癌细胞ECC-1高迁移率族蛋白B1基因表达的影响[J].湖北医药学院学报,2016,35(4):342-344,349,4.
