湖北医药学院学报2016，Vol.35Issue(4)：355-361,7.DOI:10.13819/j.issn.1006-9674.2016.04.006
UPLC-MS/MS法评价不同绿茶膳食补充剂主要成分特征
Characterization of the Major Components in Different Green Tea Dietary Supplements using HPLC-MS/MS Method
摘要
Abstract
Objective To compare the contents of major active component in different green tea dietary supplements,so as to provide basis for product quality control.Methods UPLC-MS/MS and co-elution with standard compounds were employed to identify the major components.An HPLC method was used to quantify the identified components in different supplements.Hierarchical cluster analysis(HCA) and principal component analysis(PCA) were used to evaluate the variability of these products.Results Catechin,epigallocatechin,caffeine,epicatechin,epigallocatechin-3-gallate,gallocatechin gallate,and epicatechin-3-gallate were identified as the major components in 12 different green tea supplements purchased from local store or internet.Quantitative analysis results showed that the contents of these components were highly variable across products.PCA and HCA revealed that the 12 products used in this study could be divided into four groups based on the contents of the major components per daily dose.Conclusion This study suggested that the quality of different green tea supplements are highly variable,which most likely lead to variable biological effects.Standardization of green tea supplement is necessary to derive more consistent potential benefits.关键词
绿茶膳食补充剂/质量控制/主成分分析/层序聚类分析Key words
Green tea supplement/Quality control/Principal component analysis/Hierarchical cluster analysis引用本文复制引用
胡培,杨光义,张晨宁..UPLC-MS/MS法评价不同绿茶膳食补充剂主要成分特征[J].湖北医药学院学报,2016,35(4):355-361,7.基金项目
湖北省教育厅优秀中青年科技创新团队项目(T201414) （T201414）
