湖北医药学院学报2016，Vol.35Issue(4)：362-365,370,5.DOI:10.13819/j.issn.1006-9674.2016.04.007
初发2型糖尿病患者端粒长度与胰岛功能间关系
Relationship between Telomere Length and Pancreatic Islet β-cell Function in Patients with Newly-diagnosed Type 2 Diabetes Mellitus
摘要
Abstract
Objective To investigate the relationship between telomere length change of peripheral blood leukocyte(PBL-TL) and β-cell function in patients with newly diagnosed type 2 diabetes mellitus(T2DM).Methods Total 197 newly diagnosed hospitalized T2DM patients were randomlv divided into intensive insulin treatment group(insulin group,n =99) and oral drug treatment group(oral group,n=98).The levels of fasting blood glucose(FBG),2 h postprandial glucose(2hPG),glycosylated hemoglobin (GHbA 1 c) and the indexes of islet cell functions including fasting insulin (FINS),2 h postprandial insulin(2hINS),HOMA-β,insulin resistance index(HOMA-IR) were detected and compared between two groups.After isolation of the DNA,PBL-TL from all the patiens,real-time quantitative PCR was used to determine the telomere length.The changes of islet β-cell function was compared before and after treatmnent.Another 70 cases of healthy volunteers were selected as control group.Results After treatment,the levels of FPG,2hPG,GHbA1c and Homa-IR in all patients were significantly decreased,while the levels of FINS,2hINS and Homa-β were significantly increased,espicially 2hINS and Homa -β in the insulin group.The telomere length of PBL-TL in patients with T2DM was shorter than that of health control group,but the length was increased in both insulin group and oral group,especially after insulin intensive treatment,the length ahnost recovered to the control group level in insulin group.When selecting the telomere length before treatment as dependent variable and FPG,FINS,2hINS,GHbA1C,Homa-IR,Homa-β before treatment as independent variables,the multiple linear regression results demonstrated that telomere length was only negatively correlated with age,but when selecting the difference of telomere length before and after treatment as dependent variable,the change of telomere length was positively correlated with the Homa-β(r=0.207),that was to say,the more elongation the telomere length,the higher the Homa-β was.Conclusion Short-term insulin intensive treatment could effectively improve the β-cell function probably by elongating the telomere length in patients with newly diagnosed T2DM,which was superior to the effect treating by oral drug.关键词
2型糖尿病,初发/β细胞功能/端粒长度/强化治疗Key words
Type 2 diabetes/Islet β-cell function/Telomere length/Intensive therapy引用本文复制引用
黄成虎,饶思维,蔡俊玮,李雪峰..初发2型糖尿病患者端粒长度与胰岛功能间关系[J].湖北医药学院学报,2016,35(4):362-365,370,5.基金项目
湖北医药学院立项课题(2009QDJ34) （2009QDJ34）
