湖北医药学院学报2016，Vol.35Issue(6)：527-530,546,5.DOI:10.13819/j.issn.1006-9674.2016.06.001
MRPS23shRNA对乳腺癌细胞Walker256增殖凋亡的作用及机制
Effects and Mechanisms of MRPS23-targeted shRNA on Proliferation and Apoptosis of Breast Cancer Cell Walker256
摘要
Abstract
Objective To investigate the effects and mechanisms of mitochondrial ribosomal proteins (MRPS23)-targeted shRNA on the proliferation and apoptosis of breast cancer cells Walker256.Methods The shRNA targeting against MRPS23 (shMRPS23) or non-silence sequence (shCtrl) was subcloned into lentivirus vector that containing green fluorescent protein (GFP) DNA sequence.Rat breast cancer cells Walker256 were respectively transfected with lentivirus (shCtrl,shMRPS23) or PBS (Control) for 48 h,so as to confirm the efficiency of transfection and gene silencing.The proliferation of cells was analyzed by MTT assay.The cell apoptosis was quantified TUNEL staining.The expression of MRPS23 and apoptosis-associated genes in Walker256 were detected by RT-PCR and Western blot in different groups.Results Compared with controls,the expression of MRPS23 was significantly inhibited by shMRPS23 in Walker256 cells.MRPS23 shRNA could inhibit the proliferation of breast cancer cell and significantly increased apoptotic rate (P＜0.05).QPCR and western blot demonstrated that shMRPS23 could increase the expression of p53 and p21 WAF1/CIP1.Conclusion Lentivirus mediated MRPS23 shRNA can inhibit the proliferation of breast cancer cells,which may be related with the activation of p21 WAF1/CIP1.Additionally,the p53-dependent apoptotic pathway was induced by shMRPS23.关键词
MRPS23/shRNA/乳腺癌细胞/细胞增殖/凋亡Key words
MRPS23/shRNA/Breast cancer cells/Cell proliferation/Apoptosis引用本文复制引用
高燕,李伏燕,李薇,陈义加,吴瑞敏,周红,杨怡,裴之俊..MRPS23shRNA对乳腺癌细胞Walker256增殖凋亡的作用及机制[J].湖北医药学院学报,2016,35(6):527-530,546,5.基金项目
国家自然科学基金青年项目(81401447) （81401447）
十堰市科技局指导项目(2016Y16) （2016Y16）
湖北医药学院重点专科建设项目 （）
