湖北医药学院学报2016，Vol.35Issue(6)：531-535,5.DOI:10.13819/j.issn.1006-9674.2016.06.002
利用体外合成mRNA方法研究Betatrophin对糖代谢关键基因表达的影响
Study on the Molecular Mechanism of Betatrophin Regulated Glucose Metabolism by Synthesis of Betatrophin mRNA in vitro
摘要
Abstract
Objective To study the molecular mechanism of betatrophin regulating glucose metabolism in HepG2 cell by synthesis of betatrophin Mrna in vitro.Methods Using the synthetic betatrophin Mrna plasmid as template,transfected synthetic betatrophin Mrna into HepG2 cell and detected the expression of betatrophin protein in cells by using immunofluorescence and Western blot,then analyzed the effect of betatrophin over-expression on glucose metabolism related genes expression by Qpcr and Western blot.Results Betatrophin Mrna plasmid was successfully constructed,the synthetic betatrophin Mrna in vitro could be efficiently transfected into HepG2 cells and correctly expressed betatrophin protein,the high expression of betatrophin could up regulate the key glucose metabolism gene Glut2 expression.Conclusion This experiment confirmed that betatrophin may affect theglucose metabolism through regulating the expression of Glut2,which lays a foundation for further study on the mechanism of molecular of betatrophin on the regulation of glucose metabolism.关键词
Betatrophin/HepG2细胞株/糖代谢/Glut2Key words
Betatrophin/HepG2 cells/Glucose metabolism/Glut2引用本文复制引用
汪兰,付红霞,郭兴荣..利用体外合成mRNA方法研究Betatrophin对糖代谢关键基因表达的影响[J].湖北医药学院学报,2016,35(6):531-535,5.基金项目
2016年湖北省自然科学基金面上项目(2016CFB408) （2016CFB408）
国家自然科学基金应急管理项目(81641028) （81641028）
湖北省卫生计生委一般项目(WJ2015MB223) （WJ2015MB223）
2015年湖北医药学院药学院本科生科研训练计划(SRTP)项目 （SRTP）
