湖北医药学院学报2016，Vol.35Issue(6)：579-581,585,4.DOI:10.13819/j.issn.1006-9674.2016.06.012
哌立福新对裸鼠皮下移植瘤的抑制作用
Inhibitory Effect of Perifosine on Growth of Subcutaneously Transplanted Tumor in Nude Rats
金灵莉 1卢光新 1周春芳 1吕飞 1钱颖2
作者信息
- 1. 湖北医药学院附属人民医院消化内科,湖北十堰442000
- 2. 华中科技大学同济医学院附属协和医院消化内镜中心,湖北武汉430022
- 折叠
摘要
Abstract
Objective To investigate the inhibitory effects of perifosine on the growth of subcutaneously transplanted tumor in nude rats and explore its possible mechanism.Methods The model of human esophageal cancer Ecal09 cellstransplanted subcutaneous was established in nude rats,and then divided into control group,perifosine group,cisplatin group and perifosine combined cisplatin group,the growth of tumor in all rats were observed.The apoptosis of tumor tissues were detected by TUNEL method,the expression of Akt and mTOR protein were examed by Western blot.Results Perifosine or cisplatin alone treatment could inhibit the growth of tumors(P＜0.05),the inhibitory rate increased significantly in the group of perifosine combined cisplatin (P＜0.01).The results of TUNEL method indicated that perifosine could induce the apoptosisof Eca109 cells in vivo(P＜0.05),and Western blot results showed that perifosine could inhibit the activity of Akt/mTOR of signaling pathway.Conclusion Perifosine combined cisplatin could inhibit the growth of subcutaneously transplanted tumor in nude rats,which is related to inhibiting Akt/mTOR signaling pathway.关键词
食管癌/荷瘤大鼠/哌立福新Key words
Esophageal carcinoma/Tumor bearing rat/Perifosine引用本文复制引用
金灵莉,卢光新,周春芳,吕飞,钱颖..哌立福新对裸鼠皮下移植瘤的抑制作用[J].湖北医药学院学报,2016,35(6):579-581,585,4.
