湖北医药学院学报2017，Vol.36Issue(1)：1-5,封2,6.DOI:10.13819/j.issn.1006-9674.2017.01.001
低温联合褪黑素降低小鼠局灶性脑缺血再灌注损伤
Hypothermia Combined with Melatonin Decreases Reperfusion Injuries of Mouse Focal Cerebral Ischemia
摘要
Abstract
Objective To explore the protective effects of physical hypothermia (PH) combined with melatonin (MLT) on cerebral ischemia-reperfusion injuries.Methods An in vitro oxidative stress model of mouse primary cortical neuron (PCN) was prepared using hydrogen peroxide (H2O2),and a mouse model of middle cerebral artery occlusion-reperfusion was prepared using suture embolization method.Both cellar and animal model studies included PH (34 ℃) group,melatonin group,PH (34 ℃) plus melatonin group.The protective effect on PCN was evaluated by 4',6-diamidino-2-phenylindole (DAPI) staining and lactate dehydrogenase (LDH) activity assay,and MCAO-reperfused animals were evaluated by 2,3,5-triphenyltetrazolium chloride (TTC) staining and neurological behavior scoring.Results The relative LDH activities of PCN in H2O2,PH,MLT,PH plus MLT groups were 172.8％±12.1％,150.3％±4.36％,141.6％±9.3％ and 117.2％±8.2％ respectively,which had significant difference from each other (F=33.10,P=0.000).In comparison with vehicle group,the relative brain infarct volumes of HT,MLT and combination groups were decreased by 14.7％,26.8％,56.9％,respectively.The neurobehavioral scoring of three treatment groups were also better than vehicle group.Conclusion The present results indicated that both HT and MLT pretreatment had protective effects on ischemia-reperfusion brain injuries.While combining HT with MLT could provide better protection.关键词
低温/褪黑素/缺血再灌注/小鼠/中脑动脉阻塞Key words
Hypothermia/Melatonin/Ischemia-reperfusion/Mouse/Middle cerebral artery occlusion引用本文复制引用
陈武,李毅,段发兰,李国玲,陈琴华,张正洪..低温联合褪黑素降低小鼠局灶性脑缺血再灌注损伤[J].湖北医药学院学报,2017,36(1):1-5,封2,6.基金项目
国家自然科学基金项目(81344438)资助 （81344438）
