湖北医药学院学报2017，Vol.36Issue(1)：6-9,14,封3,6.DOI:10.13819/j.issn.1006-9674.2017.01.002
应用凝集素芯片检测胃癌细胞膜表面糖链表达
Detection of the Expression of Glycan Chain on the Cell Membrane of Gastric Cancer Cells by Lectin Array
摘要
Abstract
Objective To detect the distribution pattern of glycan chains on the cell membrane by lectin array and analyze the correlation between differential glycans and the invasion/metastasis of gastric cancer cells (AGS,SGC-7901).Methods Cells were collected with trypsin digestion and labeled with a fluorescent labeling agent.Fluorescence-labeled cells were thencaptured by lectin sites on the chip based on its binding affinity to specific glycan chains.A confocal Confocal laser scanner was used to detect the expression of cell surface glycan chains.Lectin flow cytometry was used for validation.Cell invasion andmetastasis were detected by transwell and wound healing assays.Resuts No significant difference was found in fluorescent signals onmost lectin sites between AGS and SGC-7901 cells.Compared with SGC-7901 cells,LEL and MAL Ⅱ signals were stronger in AGS cells.Mannose,sialic acid,N-acetylglucosamine/glucose,and N-acetylgalactosamine/galactose were expressed in these two kinds of cells.Polylactosamine and α2-3 sialic acid were highly expressed in AGS cells.The ability of invasion and metastasis of AGS cells was stronger than that of SGC-7901 cells.Conclusion Gastric cancer cells with high invasion/metastatic potential express the characteristic polylactosamine and cα2-3 sialic acid.The lectin array-based detection of cell membrane glycan profile will help to screen cancer-related glycan markers thereby assisting the diagnosis and treatment of cancer.关键词
胃癌/细胞膜/凝集素芯片/糖链表达Key words
Gastric cancer/Cell membrane/Lectin array/Glycan profiling引用本文复制引用
董晓霞,王莹莹,孟璐,沈力..应用凝集素芯片检测胃癌细胞膜表面糖链表达[J].湖北医药学院学报,2017,36(1):6-9,14,封3,6.基金项目
国家自然科学基金青年科学基金项目(81502666) （81502666）
湖北省教育厅优秀中青年人才项目(Q20162115) （Q20162115）
