湖北医药学院学报2017，Vol.36Issue(1)：10-14,5.DOI:10.13819/j.issn.1006-9674.2017.01.003
肺炎克雷伯菌CRP调控子对细菌毒力影响与机制研究
The Role of CRP Regulator in the Virulence of Klebsiella Pneumoniae and the Pathogenesis
摘要
Abstract
Objective Understanding the effect of CRP regulator in the virulence of Klebsiella pneumoniae and the role of it in the virulence factor phosphotransferase system (PTS).Methods Construction the cAMP receptor protein (CRP) gene (crp gene) deletion mutant strain and the crp gene complemented strain and study the effect of crp gene in the virulence in mouse model.The regulation roles of CRP infrwC gene,the EIIC encoded gene of PTS,were assessed using qRT-PCR and lacZ reporter.Electrophoretic mobility shift assay (EMSA) was used to study the mechanism of CRP infrwC.Results The LD50 values was ＜100,3.2×105,and 4.5×104 CFUs for WT,Acrp,and C-crp,respectively.The expression offrwC,the fructose-specific enzyme IIC of fructose phosphotransferase system (PTS),was repressed in the mutant.EMSA show CRP can directly bind to the promoter offrwC.Conclusion pneumoniae CRP regulator was required for lethality in mouse and CRP could control the virulence through acting on fructose phosphotransferase system directly.关键词
肺炎克雷伯菌/crp基因/毒力/磷酸转移酶系统Key words
Klebsiella pneumoniae/crp gene/Virulence/Phosphotransferase system引用本文复制引用
范金明,欧琴,林迪斯,徐丽,李蓓..肺炎克雷伯菌CRP调控子对细菌毒力影响与机制研究[J].湖北医药学院学报,2017,36(1):10-14,5.基金项目
湖北省教育厅优秀中青年科技创新团队计划项目(T201612) （T201612）
