湖北医药学院学报2017，Vol.36Issue(1)：24-27,封3,5.DOI:10.13819/j.issn.1006-9674.2017.01.006
异丙酚对大鼠心肌缺血再灌注损伤的保护作用及iNOS蛋白的表达研究
Protective Effects of Propofol on Myocardial Ischemia Reperfusion Injury and the Study on iNOS Protein Expression in Rats
周丹 1胡强夫 2黄维勤3
作者信息
- 1. 武汉亚洲心脏病医院心外科,湖北武汉430022
- 2. 郑州大学第五附属医院麻醉科,河南郑州450052
- 3. 武汉亚洲心脏病医院麻醉科,湖北武汉430022
- 折叠
摘要
Abstract
Objective This study aims to explore the pathogenesis of myocardial ischemia reperfusion injury (IRI) and the treatment method.Methods The myocardial Ischemia-reperfusion rat model was established in this study.They were divided into three groups:sham operation (SO) group,IRI control (C) group and IRI with propofol (A) group (n=9).Myocardial infarct size was compared with the HE staining method.TUNEL assay was used to detect cell apoptosis.Changes in the expression of iNOS were detected using real-time PCR and Western blotting methods.FResults The myocardial infarct size of control (C) group and the propofol (A) group was (53.03_±8.90)％ and (34.73±7.20)％ respectively,there were significant difference between them (P＜0.01).The apoptotic index of two groups was 0.21-±0.02 and 0.31 ±0.05,with statistical significant difference (P＜0.05).The expression levels of iNOS in the propofol group were reduced significantly (P ＜0.05).Conclusion The levels of iNOS increased in IRI rats,suggesting that the severity of myocardial failure may be correlated with iNOS;propofol can specifically inhibit iNOS and thus protect the myocardial function.关键词
异丙酚/缺血-再灌注损伤/诱导型一氧化氮合酶Key words
Propofol/Ischemia reperfusion injury/Iinducible nitric oxide synthase引用本文复制引用
周丹,胡强夫,黄维勤..异丙酚对大鼠心肌缺血再灌注损伤的保护作用及iNOS蛋白的表达研究[J].湖北医药学院学报,2017,36(1):24-27,封3,5.
