湖北医药学院学报2017，Vol.36Issue(1)：28-35,封4,9.DOI:10.13819/j.issn.1006-9674.2017.01.007
葫芦素B逆转人乳腺癌细胞MCF-7/Dox多柔比星耐药的作用及机制研究
Effects and Mechanisms of Cucurbitacin B on Reversal of the Chemoresistance to Human MCF-7/Dox Breast Cancer Cells
摘要
Abstract
Objective To explore the effects and mechanisms of natural compound (Cucurbitacin B,CuB) on reversing drug resistance to doxorubicirn (Dox)-resisted human breast cancer (MCF-7/Dox) cells.Methods Trypan blue dye staining,MTT and colony formation assay were used to detect the inhibitory effects of CuB on the growth of MCF-7/Dox cells.DAPI staining and Western blot were used to investigate the mechanisms of CuB on reversing Doxorubicin-resisted MCF-7/Dox cells.FResults CuB inhibited MCF-7/Dox cell proliferation,cell viability,and reversed chemoresistance to doxorubicin.CuB down-regulated the expression of P-glycoprotein,multidrug resistance-associated protein 1,and Cyclin D1.CuB also induced caspase-dependent apoptosis,and decreased phosphorylation of Akt (pAkt).The suppression of pAkt was mediated by CuB-induced activation of protein phosphatase 2A (PP2A) through the suppressing CIP2A.Conclusiorn The CIP2A/PP2A/Akt pathway may mediate the reversal effect of CuB on Doxorubicin-resisted MCF-7/Dox cells.关键词
葫芦素B/乳腺癌/CIP2A/PP2A/多药耐药/凋亡Key words
Cucurbitacin B/Breast cancer/CIP2A/Multi-drug resistance/Apoptosis引用本文复制引用
蔡芬,段超,黄秋月,袁晓宁,季娟丽,张特,张亮..葫芦素B逆转人乳腺癌细胞MCF-7/Dox多柔比星耐药的作用及机制研究[J].湖北医药学院学报,2017,36(1):28-35,封4,9.基金项目
湖北医药学院研究生启动基金项目(2015QDJZR16) （2015QDJZR16）
2016年度国家大学生创新创业训练项目(201610929001) （201610929001）
