中风与神经疾病杂志2017，Vol.34Issue(4)：292-294,3.
柚皮苷对脑缺血再灌注损伤的保护作用
The protective effect of naringin on cerebral ischemia reperfusion injury
摘要
Abstract
Objective To study the protective effects of naringin on rats with focal cerebral ischemia and reperfusion and whether astrocyte participates in this effect.Methods Naringin group was pretreated intragastricly for consecutive 7 days before ischemia.Middle cerebral artery was occluded for 2 h and then reperfused for 24 h.The number of apoptotic cells was detected by TUNEL staining,and pathological changes were observed by hematoxyli-eosin (HE) staining.Immunofluorescent staining was used to observe astrocytic marker (glial fibrillary detection acidic protein,GFAP) and connexin43 (Cx43).Results Compared with ischemia group,pretreatment with naringin reduced the number of apoptotic cells (P<0.05),and significantly attenuated cell injury.Immunofluorescent staining showed that naringin attenuated astrocytic activation,and decreased the expression of Cx43 (P<0.05).Conclusion Naringin pretreatment can effectively attenuated cerebral ischemia reperfusion injury,and the protective effects are closely related with Cx43 in astrocytes.关键词
柚皮苷/缺血再灌注/星形胶质细胞/Cx43Key words
Naringin/Ischemia reperfusion/Astrocytes/Cx43分类
医药卫生引用本文复制引用
刘微,张洋,郭建超,练鹏影,吴敏华,丁文婷..柚皮苷对脑缺血再灌注损伤的保护作用[J].中风与神经疾病杂志,2017,34(4):292-294,3.基金项目
国家自然科学基金资助项目(No.81202817,81673772) （No.81202817,81673772）
广东省高等学校优秀青年教师培养项目(No.Yq2013041) （No.Yq2013041）
高等学校博士学科点专项科研基金(No.20124425120011) （No.20124425120011）
