中国病理生理杂志Issue(4)：620-626,7.DOI:10.3969/j.issn.1000-4718.2017.04.008
高糖通过下调PGC-1α激活NFAT并促进足细胞凋亡
High glucose-activated NFAT promotes podocyte apoptosis by down-regulation of PGC-1
陈恩平 1杜丽根 1邬银伟 1叶翠媚 1罗权芳1
作者信息
- 1. 深圳市龙岗区第二人民医院, 广东 深圳 518112
- 折叠
摘要
Abstract
AIM:To explore whether down-regulation of peroxisome proliferator-activated receptor γ coactivator (PGC)-1α induces podocyte apoptosis and its mechanism.METHODS:The podocytes were cultured under high glucose (HG) condition and the cell apoptosis was analyzed by flow cytometry.The methods of real-time PCR and Western blot were used to analyze the mRNA and protein expression of related molecules in the control, HG-treated or siRNA-treated podocytes.RESULTS:The expression PGC-1α at mRNA and protein levels was significantly decreased in HG-injured podocytes.Down-regulation of PGC-1α expression in vitro by siRNA resulted in podocyte apoptosis.The nuclear protein expression of nuclear factor of activated T-cells (NFAT) was significantly increased in HG injured podocytes, indicating the NFAT activation.Down-regulation of PGC-1α expression also decreased the nuclear protein expression of NFAT.Moreover, silencing of NFAT expression by siRNA significantly abolished PGC-1α deficiency-induced podocyte apoptosis.CONCLUSION: Down-regulation of PGC-1α induces podocyte apoptosis.NFAT mediates PGC-1α deficiency-induced podocyte apoptosis.关键词
高糖/足细胞/细胞凋亡/过氧化物酶体增殖物激活受体γ共激活因子1α/活化T细胞核因子Key words
High glucose/Podocytes/Apoptosis/Peroxisome proliferator-activated receptor γ coactivator-1α/Nuclear factors of activated T-cells分类
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陈恩平,杜丽根,邬银伟,叶翠媚,罗权芳..高糖通过下调PGC-1α激活NFAT并促进足细胞凋亡[J].中国病理生理杂志,2017,(4):620-626,7.
