中国病理生理杂志Issue(4)：688-693,6.DOI:10.3969/j.issn.1000-4718.2017.04.018
SLeX对肝癌HepG2细胞迁移和侵袭的影响
Effects of SLeX on invasion and migration of HepG2 cells
摘要
Abstract
AIM: To investigate the expression and effects of sialyl Lewis X (SLeX) on the invasion and migration of human hepatocellular carcinoma HepG2 cells.METHODS: The expression of α1,3-fucosyltransferase VII (FUT7) in HepG2 cells and L-02 cells was detected by RT-qPCR and Western blot.The SLeX expression in HepG2 cells and L-02 cells was determined by Western blot and immunocytochemical staining.The invasion and migration abilities of the treated cells were evaluated by Transwell assay.RESULTS: The expression of FUT7 and SLeX in the HepG2 cells, but not in the L-02 cells, was observed.The invasion rates of the HepG2 cells treated with SLeX monoclonal antibody at 0.05, 0.5 and 5 mg/L were significantly decreased as compared with control group (P<0.05).The migration ability of the HepG2 cells treated with SLeX monoclonal antibody at 0.05, 0.5 and 5 mg/L was also significantly reduced as compared with control group (P<0.05).The invasion rate and migratory cell number were significantly different between any 2 groups in the HepG2 cells treated with SLeX monoclonal antibody at 0.05, 0.5 and 5 mg/L (P<0.05).CONCLUSION: HepG2 cells express SLeX.SLeX is closely related to the migration and invasion abilities of the HepG2 cells.关键词
唾液酸化路易斯寡糖X/肝细胞癌/HepG2细胞/细胞迁移/细胞侵袭Key words
Sialyl Lewis X/Hepatocellular carcinoma/HepG2 cells/Cell migration/Cell invasion分类
医药卫生引用本文复制引用
武文娟,崔灿,康品方,石玉荣,耿英华..SLeX对肝癌HepG2细胞迁移和侵袭的影响[J].中国病理生理杂志,2017,(4):688-693,6.基金项目
安徽省教育厅省自然科学基金资助项目(No.KJ2011B098 （）
No.KJ2014A162 （）
No.KJ2017A234) （）
国家级大学生创新训练计划(No.201310367041) （No.201310367041）
