中国感染控制杂志2017，Vol.16Issue(4)：326-329,4.DOI:10.3969/j.issn.1671-9638.2017.04.009
182株儿童患者肺炎链球菌的分子生物学鉴定及分型
Molecular identification and typing of 182 strains of Streptococcus pneumoniae isolated from children
摘要
Abstract
Objective To type Streptococcus pneumoniae(S.pneumoniae) isolated from children, and provide scientific basis for the correct selection of S.pneumoniae vaccine.Methods 182 strains of S.pneumoniae were collected from Children's Hospital of Hebei Province in 2014, species of strains were identified by polymerase chain reaction (PCR), types of strains were analyzed with multiplex PCR.Results PCR detection showed that cpsA gene amplification of 182 strains were all positive;multiplex PCR detection revealed that except 8 strains were not typed, the main types of the remaining 174 strains were 19 F (n=68, 37.36%), 19A(n=33, 18.13%), and 6A/6B (n=26,14.28%), the other types were 35B, 14, 6C/6D, 23F, 15B/15C, and so on.Conclusion The main types of 182 strains of S.pneumoniae are 19 F, 19A, and 6A/6B, which provide scientific basis for the correct selection of S.pneumoniae vaccine for this province.关键词
肺炎链球菌/多重PCR/菌型鉴定/种属鉴定/cps基因Key words
Streptococcus pneumoniae/multiplex polymerase chain reaction/bacterial typing/species identification/cps gene分类
医药卫生引用本文复制引用
郭映辉,何宝花,王颖童,贾肇一,王茜,李贵霞,张文超,孙印旗,陈素良..182株儿童患者肺炎链球菌的分子生物学鉴定及分型[J].中国感染控制杂志,2017,16(4):326-329,4.基金项目
河北省科技支撑计划项目(14277733D) （14277733D）
