局解手术学杂志2017，Vol.26Issue(4)：235-239,5.DOI:10.11659/jjssx.08E016058
电击死心脏窦房结组织纤维连接蛋白的表达
Expression of fibronectin protein levels in sinoatrial node tissues of hearts following electrocution
摘要
Abstract
Objective To study the protein levels of fibronectin (Fn) in the sinoatrial node tissues of hearts caused by electric shock death ,and to provide a new theoretical basis for the diagnosis of electric shock death and the identification of death time.Methods Using immunohistochemical technique(S-P method) to detect protein levels of 15 cases of human sinoatrial node following electrocution(human experimental group),15 cases of human sinoatrial node with severe traumatic cerebral injury(human control group),35 cases of rabbit sinoatrial node following electrocution(rabbit experimental group ,divided into 7 groups according to different sampling time: 0 h ,1 h ,3 h ,6 h ,12 h,24 h.48 h),and 35 cases of rabbit sinoatrial node with broken neck executed(rabbit control group ,divided int0 7 groups according to different sampling time :0 h,1 h.3 h,6 h.12 h.24 h,48 h).Results Fn positive expression rate in human experimental group was 100% while it was 6.67% in the human control group,and the differences were statistically significant (P < 0.01 ).The Fn expression can be seen after electric shock immediately in the rabbit experimental group,and there was statistical difference compared with the rabbit control group (P < 0.05).Distribution of Fn expression became more and more widely after 3 hours t0 12 hours in the rabbit experimental group ,and there was significant difference compared with the rabbit control group(P < 0.01).The Fn expression began to decrease 24 hours after electrocution,ancl it still had statistical significance 48 hours after electrocution compared with the rabbit control group (P < 0.05).Conclusion Electric shocks significantly enhanced the expression of Fn protein levels in the sinoatrial node tissues , and optical density of Fn positive expression has a regularity change along with the extended time of death.These results could provide a theoretical reference basis for forensic diagnosis of electrocution and identification of death time.关键词
电击死/窦房结/纤维连接蛋白/免疫组织化学Key words
electrocution/sinus node/fibronectin/immunohistochemistry分类
医药卫生引用本文复制引用
李贵花,王星,刘德斌,李志军,张少杰..电击死心脏窦房结组织纤维连接蛋白的表达[J].局解手术学杂志,2017,26(4):235-239,5.基金项目
国家自然科学基金(81260269 （）
81560348) （）
