局解手术学杂志2017，Vol.26Issue(4)：240-243,4.DOI:10.11659/jjssx.01E017081
Notch1信号通路对人牙周膜干细胞应力分化过程的调控
Role of Notch1 signal pathway in osteogenic differentiation of human periodontal ligament stem cells
周述作 1秦凡 1崔嘉玺 1董立 2周继祥1
作者信息
- 1. 第三军医大学西南医院口腔科,重庆 400038
- 2. 重庆大学生物医学工程学院,重庆 400044
- 折叠
摘要
Abstract
Objective To explore the effect of Notch1 signal pathway on the osteogenic differentiation in human periodontal ligament stem cells(PDLSCs) under dynamic strain.Methods PDLSCs were separated from freshly extracted teeth then identified and prolifed.Notch1 signal pathway was regulated by chemicals.Dynamic strains were applied to PDLSCs with the tension plus system.Then Notch1 signal pathway key factor Notch intracellular domain(NICD),osteoblastic related indexes alkaline phosphatase(ALP) and bone morphogenetic proteins 2 (BMP2) were detected by western blot expression.The deformation rate of stress parameters was 0 to 12%,and the frequency was 0.1 Hz.The loading time was 0 h,6 h,12 h and 24 h.Results As Notch1 signal pathway was activated,the expression of osteogenic markers ALP and BMP2 both reduced (P<0.05).On the contrary, the expression of osteogenic markers ALP and BMP2 both increased obviously (P<0.05) as Notch1 signal pathway was inhibited.Conclusion The activated Notch1 signal pathway will inhibit osteogenic differentiation of PDLSCs under dynamic tensile.关键词
Notch1/人牙周膜干细胞/Jagged1/DAPT/动态张应力/成骨分化Key words
Notch1 signal pathway/human periodontal ligament stem cells/Jagged1/DPAT/dynamic tensile/osteoblast differentiation分类
医药卫生引用本文复制引用
周述作,秦凡,崔嘉玺,董立,周继祥..Notch1信号通路对人牙周膜干细胞应力分化过程的调控[J].局解手术学杂志,2017,26(4):240-243,4.
