局解手术学杂志2017，Vol.26Issue(4)：244-247,4.DOI:10.11659/jjssx.01E017040
不同剂量利福平致小鼠肝损伤特点的研究
Research on characteristics of liver injury induced by rifampicin in mice
摘要
Abstract
Objective To observe the symptom and characteristics of liver injury induced by different dosage of long-term administration of rifampicin(RIF) in mice.Methods Twenty-four healthy female ICR mice were randomly divided into 4 groups(6 mice in each group):control group,low dosage group,medium dosage group and high dosage group.The four groups were treated with 0,100,200,400 mg·kg-1·d-1 RIF respectively for 2 weeks.Mice blood and liver tissue samples were collected at 6 hours after the last administration for serological test and liver histological observation.Results No mice died before execution.The TBA,DBIL and TBIL of high dosage group all increased compared with the control group, but the ALT,AST and ALP showed no obvious change.The TBA and DBIL of medium dosage group increased compared with the control group, while the TBIL,ALT,AST and ALP showed no obvious change.In the low dosage group,there was no obvious change in terms of TBA,DBIL,TBIL,ALT,AST,and ALP compared with the control group.Obvious pathological change occured in the liver of mice in all the experimental groups.HE staining showed edema and feather steatosis in liver cells, accompanied by a large number of inflammatory cells infiltration and a few sporadic cholestasis.With the increasing of RIF dosage,the liver pathological change became more obviously.In the experimental group,electron microscope showed that there were a lot of fat droplets in the liver cells wrapped slurry,and part of the capillary bile duct were slightly expanded with different electron density and irregular shape of bile sample material inside.The pathologic changes get more obvious with the increase of the concentration of rifampicin as well.Conclusion RIF could induce liver injury after 2 weeks' treatment at different dosages,mainly pathological changes included liver cell steatosis,inflammatory cell infiltration,and cholestasis.Rifampicin induced liver injury in a concentration-dependent manner.However,the mechanism of rifampicin-induced liver injury in mice needs further study.关键词
利福平/肝损伤/胆汁淤积/小鼠/抗结核药物/肝毒性Key words
rifampicin/liver injury/cholestasis/mice/antituberculosis drugs/hepatotoxicity分类
医药卫生引用本文复制引用
张刚,陈磊,陈静,柴进,汤旭东,陈文生..不同剂量利福平致小鼠肝损伤特点的研究[J].局解手术学杂志,2017,26(4):244-247,4.基金项目
国家自然科学基金面上项目(81470850) （81470850）
