中国临床药学杂志2017，Vol.26Issue(2)：110-115,6.
3种苯二氮(卓)类药物在海马神经细胞模型中的吸收及利用度比较
Comparative study on the absorption and utilization of three kinds of benzodiazepines in hippocampal neuronal cells model
摘要
Abstract
AIM To establish a method for quantitative determination of diazepam,clonazepamand,lorazepam inside and outside of hippocampal neuronal cells,and study the absorption and utilization of the three components in cell model.METHODS The quantitative method of three kinds of benzodiazepines by HPLC-CAD was established.The samples were separated on C18 column(250 mm ×4.6 mm,5 μm) with the mobile phase consisted of acetonitrile-water(44:56),the column temperature was 30℃,the flow rate was 1.0 mL · min-1,the detection wavelength was 254 nm,and the external standard method was used.RESULTS Three benzodiazepines could be well separated from the other components of the culture medium and cells.In the range of 1.83-183.16 mg · L-1 of diazepam,1.81-190.04 mg· L-1 of clonazepam and 1.74-173.56 mg · L-1 of lorazepam respectively,there were in good linear relationship,and the average recovery rate was 99.99％,100.42％ and 99.98％ respectively.The hippocampal neuronal cells were treated by three benzodiazepines for 24 h.The average absorption rate of normal control group was 45.11％,32.60％ and 29.73％,and the average absorption rate of model group was 31.30％,25.98％ and 21.81％ respectively.The average utilization rate of normal control group was 72.98％,73.16％ and 74.90％,and the average utilization rate of model group was 78.89％,83.04％ and 85.01％ respectively.CONCLUSION The absorption of normal control group is higher than model group,while the utilization of model group is higher than normal control group.The method is accurate and sensitive,and can provide a reference for the study of drug dynamics in hippocampal nerve cell.关键词
苯二氮(卓)类/含量测定/海马神经细胞/HPLC-CADKey words
benzodiazepines/content determination/hippocampal nerve cell/HPLC-CAD引用本文复制引用
熊雪丰,肖昌钱,韩奇..3种苯二氮(卓)类药物在海马神经细胞模型中的吸收及利用度比较[J].中国临床药学杂志,2017,26(2):110-115,6.基金项目
浙江省医药卫生科技计划项目(编号2016KYA003) （编号2016KYA003）
