中国人兽共患病学报2017，Vol.33Issue(3)：228-235,8.DOI:10.3969/j.issn.1002-2694.2017.03.007
福建省1例不明原因肺炎病例中甲型H1N1流感病毒的全基因组特征分析
Complete genome analysis of influenza A(H1N1) pdm09 virus isolated from one case of pneumonia of unknown etiology (PUE) in Fujian Province, China
摘要
Abstract
To study the biological characteristics and mutations of influenza A(H1N1)pdm09 virus isolated from one case of pneumonia of unknown etiology (PUE),which would provide references for clinical treatment and disease control,the throat swab specimen from the PUE case was isolated in the Madin-Darby Canine Kidney (MDCK) cells,and then the antigenicity,pathogenicity and drug resistance of influenza A (H1N1) pdm09 virus were analyzed after sequencing.As a result,one influenza virus strain was isolated from the specimen and named as A/FujianGulou/SWL64/2016(H1N1).The similarities of nucleotide sequences and amino acids sequences compared with the vaccine strain A/California/07/2009 (H1N1) were 96.9％-98.9％ and 96.7％-99.5％,respectively.Eighteen amino acids had mutated in the HA and 4 mutations,K163Q,S185T,S203T and D222N,were involved in 3 different epitopes,which indicated that the antigenic drift had occurred in the influenza virus.The D222N mutation associated with receptor binding site made the virus infect lower respiratory tract more easily.The virus was still amantadine-resistance and oseltamivir-sensitive.In conclusion,the influenza A (H1N1) pdm09 virus in this study have occurred antigenic drift and has the molecular characterization of causing severe pneumonia,so further surveillance should be performed to prevent and control the influenza epidemic.关键词
不明原因肺炎/甲型H1N1流感/全基因组特征Key words
pneumonia of unknown etiology (PUE)/influenza A(H1N1) pdm09 virus/characteristics of complete genome分类
医药卫生引用本文复制引用
张炎华,郑奎城,翁育伟,张建明,修文琼,陈宏彬,赵琳,何文祥,朱颖,谢剑锋..福建省1例不明原因肺炎病例中甲型H1N1流感病毒的全基因组特征分析[J].中国人兽共患病学报,2017,33(3):228-235,8.基金项目
福建省自然科学基金(No.2015J01152) （No.2015J01152）
福建省医学创新课题(No.2014-CX-9)资助 （No.2014-CX-9）
福建省卫生系统中青年骨干人才培养项目(No.2015-ZQN-ZD-10) （No.2015-ZQN-ZD-10）
Supported by the Natural Science Foundation of Fujian Province,China (No.2015J01152),the Medical Innovation Subject of Fujian Province (No.2014-CX 9) and the Training Project of Young and Middle-aged Talents in Health System of Fujian Province (No.2015-ZQN-ZD-10) （No.2015J01152）
