中国实验动物学报2017，Vol.25Issue(2)：146-152,7.DOI:10.3969/j.issn.1005-4847.2017.02.006
PP1γ基因DNA甲基化在学习记忆中的作用
Effect of expression of PP1γ and DNA methylation on learning and memory in mice
摘要
Abstract
Objectives To study the role of protein serine/threonine phosphatase1γ(PP1γ) and DNA methylation in learning and memory.Methods The mice and cells were treated with 5-aza-cdR,a DNA methyltransferase(DNMT) inhibitor,and to observe the changes of ability of learning and memory and expression levels of PP1γin mice.The ability of learning and memory in mice was assessed by Morris Water maze test.The mRNA expression levels of DNMTsand PP1γ in the mouse hippocampus were determined by real-time PCR and the protein level of PP1γ was measured by Western blot.To further investigate their role,NG108-15 cell line was treated with 5-aza-cdR.Flow cytometry,xCelligence and luciferase were used to detect the effects of 5-aza-cdR on the proliferation,apoptosis andPP1γtranscription in the NG108-15 cells.Results The ability of learning and memory was enhanced in the mice after administration of 5-aza-cdR injection.The expressions of DNMTs and PP1γ were decreased in the mouse hippocampus after injection of 5-aza-cdR.On the other side,10 μmol/L 5-aza-cdR inhibited cell proliferation and decreased PP1γ transcription without inducing apoptosis.Conclusions Our data demonstrate that 5-aza-cdR inhibits the expression of PP1γ which is related to learning and memory in mice.关键词
蛋白磷酸酶1γ/5-aza-cdR/海马/学习记忆Key words
PP1γ/5-aza-cdR/Hippocampus/Learning and memory分类
生物科学引用本文复制引用
侯林,许晟迪,张柱霞,田小莉,姜树原,刘友,巴德仁贵,黄丽华,邵国..PP1γ基因DNA甲基化在学习记忆中的作用[J].中国实验动物学报,2017,25(2):146-152,7.基金项目
国家自然科学基金(Nos:81460283,81660307) （Nos:81460283,81660307）
内蒙古自然科学基金(No:2014MS0810). （No:2014MS0810）
