摘要
Abstract
Obejective To explore the protective effect of nimodipine on PC12 cell apoptosis induced by hydrogen peroxide (H2O2).Methods The cells were randomized into five groups: normal group,model group (200 μmol/L H2O2),nimodipine low-,medium-and high-dose groups (1,10,100 μmol/L nimodipne+200 μmol/L H2O2).Cell viability was measured by MTT assay.Cell apoptosis was assessed by Hoechst staining.The activity of cysteinyl aspartate specific proteinase 3 (caspase 3),caspase 9 and superoxide dismutase (SOD),the level of malonic aldehyde (MDA) were measured by colorimetry.The expression of B-cell lymphoma-2 (Bcl-2),Bcl-2 associated X protein (Bax) and p53 were detected by western blot.Results Compared with the normal group,the cell apoptosis rate,the activity of caspase 3 and caspase 9,the MDA level,and the expression of Bax and p53 were significantly increased,the cell viability,SOD activity,and expression of Bcl-2 were decreased in the model group (P<0.01).Compared with the model group,the cell apoptosis rate,the activity of caspase 3 and caspase 9,the level of MDA,and the expression of Bax were decreased;and the cell viability,SOD activity,and the expression of Bcl-2 were increased in the nimodipine low-,medium-and high-dose groups (P<0.01).Conclusions Nimodipine suppresses cell apoptosis induced by H2O2 in PC12 cells,which may be related to regulation of expression of cell apoptosis-related proteins.关键词
尼莫地平/PC12细胞/过氧化氢/凋亡Key words
Nimodipine/PC12 cell line/Hydrogen peroxide/Apoptosis分类
医药卫生
