中国比较医学杂志2017，Vol.27Issue(4)：20-25,6.DOI:10.3969.j.issn.1671-7856.2017.04.004
辛伐他汀对尾悬吊再负重大鼠长骨骨量恢复的影响
Effect of simvastatin on bone mass recovery in rats with reloading after tail-suspension
摘要
Abstract
Objective To observe the changes of bone mass in reloaded rats after tail-suspension,and the effect and mechanism of simvastatin on this process.Methods Twenty-four 5-month old rats were divided into 4 groups of 6 animals in each group: Control (CL) group without tail-suspension,unloaded (UL) group with tail-suspension for 6 weeks,other 12 rats received tail-suspension for 3 weeks,then reloaded for subsequent 3 weeks (UL+RL) or combined with simvastatin treatment (UL+RL+SIM) at a dose of 10 mg/kg/d.All rats were sacrificed 6 weeks later,and the left femur was used for examination of bone mineral density,left tibia was used for bone histomorphometry analysis,the right femur and tibia were harvested for biomechanical test,and expression levels of type I collagen by real-time PCR and Western blot,respectively.Results 1.BMD of the CL group was significantly higher than those of the other three groups (P<0.05),and was markedly lower than those in the UL+RL and UL+RL+SIM groups (P<0.05).2.The bone histomorphometry showed that BV/TV in the CL group was significantly higher than those in the other 3 groups,and the UL+RL and UL+RL+SIM groups showed a significantly higher BV/TV than that of UL group (P<0.05).The Tb.Th was significantly higher in the CL group than in the UL group.The Tb.Sp in the CL group was significantly lower than those in the other 3 groups (P<0.05).The UL+RL and UL+RL+SIM groups showed significantly lower Tb.Sp than that of the UL group (P<0.05).3.Biomechanical test showed that the maximal load and elastic modulus in the CL groups were significantly higher than those of the other three groups (P<0.05).4.Real-time PCR showed that no significant difference in the mRNA expression level of Col I was found between any two groups.5.Western blot showed that the IOD of Col I is significantly lower than that in the CL group.Conslusions Bone loss,destruction of trabecular bone micro-architecture and biomechanical properties and reduction of type 1 collagen are present in tail-suspension treated rats,which are partially restored after reloading,and this recovery process is not enhanced by simvastatin treatment.关键词
辛伐他汀/尾悬吊/骨密度/骨组织形态计量学/生物力学/I型胶原Key words
Simvastatin/Tail-suspension/Bone mineral density/Bone histomorphometry/Biomechanical test/Collagen type I.分类
医药卫生引用本文复制引用
周沛飞,胡东,张岩,刘昊,田发明,邢磊..辛伐他汀对尾悬吊再负重大鼠长骨骨量恢复的影响[J].中国比较医学杂志,2017,27(4):20-25,6.基金项目
河北省高等学校科学研究计划(QN20131007)、河北省自然科学基金(H2013209255). （QN20131007）
