中国比较医学杂志2017，Vol.27Issue(4)：26-33,40,9.DOI:10.3969.j.issn.1671-7856.2017.04.005
海马内微量注射K252a对大鼠行为学及抑郁样经典指标的影响
Changes of behavior and depression-like classic indicators after hippocampal microinjection of K252a
摘要
Abstract
Objective To study the changes of behavior and depression-like classic indicators after hippocampal microinjection of K252a,and to establish a new animal model of depression.Methods SD rats were randomly divided into five groups,namely the control group,sham group,chronic stress depression model group,hippocampal of K252a microinjection group,and hippocampal microinjection K252a plus chronic stress group.Open field experiments,sucrose consumption test,and Morris water maze behavioral assay were used to assess the behavioral changes in the rats.ELISA was used to detect the plasma monoamine neurotransmitter,radioimmunoassay was used to determine the plasma CRH,ACTH,CORT contents,and western-blotting was performed to observe the protein expression of BDNF,CREB,ERK1/2,and BCL-2 in the hippocampus.Results Compared with the control group,the amount of activity,sugar consumption,learning and memory abilities were decreased(P<0.05 or P<0.01),also the serum monoamine neurotransmitters were decreased (P<0.01),HPA axis function was improved (P<0.01),and the expression of BDNF,CREB,ERK1/2,BCL-2 decreased in the CUMS group(P<0.05 or P<0.01),but there was no significant difference in the DMSO group.Compared with the DMSO group,the activity,consumption of sucrose,learning and memory ability were significantly decreased(P<0.05 or P<0.01),while the HPA axis function was increased (P<0.05 or P<0.01),the serum monoamine neurotransmitters decreased(P<0.05 or P<0.01),and the BDNF,CREB,ERK1/2,BCL-2 expressions in the hypocampus were significantly decreased(P<0.05 or P<0.01) in the K252a group and K252a + CUMS group.Compared with the CUMS group,the K252a group and K252a + CUMS group did not show significant changes in these parameters.Compared with the K252a group,these indicators were not significantly changed in the K252a + CUMS group.Conclusions The results of behavior,hematology,and molecular biology analysis show that this model has a great similarity to the classical model of CUMS in surface validity,construct validity,and functional validity.It may provide an alternative investigative technology platform for basic research and antidepressant drug screening.关键词
抑郁症/模型,动物/K252a/神经营养障碍Key words
Depression/Models/animal/K252a/Neurotrophic disorders引用本文复制引用
孟盼,朱青,张秀丽,向韵,王宇红,杨蕙,雷昌..海马内微量注射K252a对大鼠行为学及抑郁样经典指标的影响[J].中国比较医学杂志,2017,27(4):26-33,40,9.基金项目
国家自然科学基金青年项目(81403387) （81403387）
湖南省教育厅一般项目(14C0862 （）
15C1038) （）
湖南省科技厅重点项目(2015DK3003) （2015DK3003）
中医内科学省部共建教育部重点实验室开放基金(ZYNK201503). （ZYNK201503）
