中国比较医学杂志2017，Vol.27Issue(4)：46-51,6.DOI:10.3969.j.issn.1671-7856.2017.04.008
慢病毒介导稳定敲低Smurf1细胞株的构建及对细胞迁移的影响
Establishment of two human tumor cell lines with lentivirus-mediated stably Smurf1 silencing and their effect on cell migration
摘要
Abstract
Objective To establish lentiviral expression vectors for Smurf1 silencing and assess the effects of Smurf1 silencing on cell migration.Methods HeLa and A549 cells were infected with lentiviral expression vectors for Smurf1 silencing respectively.After 7 days,the stable cell lines with Smurf1 silencing were obtained after puromycin-resistance screening,enrichment and expansion.The intracellular gene and protein levels of Smurf1 were detected by qPCR and western blot.Transwell assay was used to assess the effect of Smurf1 silencing on cell migration.Results The stable cell lines with Smurf1 silencing are constructed successfully.Silencing of Smurf1 down-regulated cell migration rate detected by Transwell assay.Conclusion Smurf1 promotes cell migration.关键词
Smurf1/慢病毒/RNA干扰/细胞迁移Key words
Smurf1/lentivirus/RNA interference/Cell migration分类
医药卫生引用本文复制引用
韦荣飞,郭静,李梦媛,朱瑞敏,杨星九,高苒..慢病毒介导稳定敲低Smurf1细胞株的构建及对细胞迁移的影响[J].中国比较医学杂志,2017,27(4):46-51,6.基金项目
协和青年基金资助,中央高校基本科研业务费专项资金资助(3332016077),中国医学科学院医学实验动物研究所基本科研业务费专项资助(2016ZX310033). （3332016077）
