中国比较医学杂志2017，Vol.27Issue(4)：56-62,7.DOI:10.3969.j.issn.1671-7856.2017.04.010
Shkbp1缺失对小鼠T淋巴细胞系亚群的影响
Effects of Shkbp1 deletion on mouse T lymphocyte subsets
摘要
Abstract
Objective Shkbp is also called Shkbp1,can competitively inhibit binding CIN85 and c-Cbl,thereby blocking the epidermal growth factor receptor (EGFR) endocytosis and degradation,to play a role in tumor promotion.This study aims to explore the changes in blood cell classification and T cell subsets in blood,bone marrow,and spleen in Shkbp1-deletion (Shkbp-1-/-) mice.Methods Shkbp-1-/-transgenic mice were identified by PCR genotyping.Blood cell classification was performed using an automatic classification system.Flow cytometry was used to detect the T lymphocyte subsets in the blood,bone marrow,and spleen of Shkbp-1-/-and control mice.Results Routine blood examination showed that neutrophils and eosinophils tended to increase and showing significant differences,and there was no significant difference in lymphocytes.The flow cytometry results showed that there was a decrease of CD4+CD8+ double positive cells and increase of bone marrow CD3+ and CD4+ cells in the control group.However,there was a decreasing trend of CD3+,CD4+,CD8+,and CD4+CD8+ cells in the spleen tissues.Conclusions Shkbp1 is involved in the maturation and differentiation of blood cells,and affects the number of immune cells.This study lays a foundation for the study of how Shkbp1 is involved in the differentiation of blood cells.关键词
Shkbp-1/T细胞亚群/CD3+/CD4+/CD8+/基因工程小鼠Key words
Shkbp-1/Leukocytes/T cell subsets/CD3+/CD4+/CD8+/CD4+CD8+lymphocytes/Genetically engineered mice分类
医药卫生引用本文复制引用
刘擎,张潇涵,仰明明,周泽启,王丽京,李江超..Shkbp1缺失对小鼠T淋巴细胞系亚群的影响[J].中国比较医学杂志,2017,27(4):56-62,7.基金项目
国家自然基金(No.81472336和No.31471290)和广东省科技计划(2015A030302086和2014A020212313)共同资助. （No.81472336和No.31471290）
