中国比较医学杂志2017，Vol.27Issue(4)：69-74,6.DOI:10.3969.j.issn.1671-7856.2017.04.012
C57BL/6J小鼠冻融胚胎移植后所得小鼠及其子代生物学特性数据测定与分析
Mensurement and analysis of biological characteristics of C57BL/6J mice and their offsprings after frozen-thawed embryo transfer of C57BL/6J mice
摘要
Abstract
Objective To investigate the changes of average body weight gain and serum biochemical indexes of C57BL/6J mice (B6 mouse) and their offspings after frozen-thawed embryo transfer of B6 mice.Methods The mice were divided into three groups in this study.In the experimental group I (E-I,30 males and 20 females),2-cell embryos after in-vitro fertilization were collected,and cryopreserved by EFS method,then obtained the offsprings after transplantation of the recovered embryos to oviduct of recipient mice (ICR mouse).In the experimental group II (E-II,26 males and 17 females),when the mice from E-I grew to maturity,the offsprings were obtained from natural mating of mice from E-I.In the control group (20 males and 20 females),the offsprings came from conventional feeding and natural mating.The three groups of mice were raised to 16 weeks old,weighing the body weight at a regular time intervals,and the serum biochemical indexes were obtained from 16-week-old mice.Then the changes of average body weight and serum biochemical indexes of the mice were analyzed.Results The average body weight of E-I mice was significantly higher than that of control group at each week-age (P<0.01).The average body weight of E-II female mice was significantly higher than that of the control group in 12-16-week old mice (P<0.01),but the average body weight of E-II male mice showed no significant differences compared with the control group except for few weeks.The serum biochemical indexes of E-I and E-II mice were changed in all items except for AST,TP and Ca.Conclusions There are some effects on the average body weight gain and serum biochemical indexes of C57BL/6J mice and their offspings after frozen-thawed embryo transfer.关键词
冻融胚胎/体增长/血清生化指标Key words
Frozen-thawed embryo/Body weight gain/Serum biochemical index/C57BL/6J mice分类
医药卫生引用本文复制引用
徐伟超,顾剑洁,葛蓉,刘丽均,徐平,芮荣..C57BL/6J小鼠冻融胚胎移植后所得小鼠及其子代生物学特性数据测定与分析[J].中国比较医学杂志,2017,27(4):69-74,6.基金项目
上海市科委科研计划项目(15140900100,14140900500). （15140900100,14140900500）
