中国动物传染病学报2017，Vol.25Issue(1)：26-31,6.
猪伪狂犬病基因缺失灭活疫苗(JS-2012-△gI/gE株)最小免疫剂量测定
DETERMINATION OF THE MINIMUM IMMUNIZATION DOSE OF SWINE PSEUDORABIES INACTIVATED VACCINE
摘要
Abstract
In order to determine the minimum immune dose of the inactivated vaccine of swine Pseudorabies (PRV JS-2012-gI/gE strains),twenty five 14-day-old piglets,seronegative for Pseudorabies virus(PRV),Porcine reproductive and respiratory syndrome virus (PRRSV),Porcine circovirus (PCV2) and Classical swine fever virus (CSFV)amibodies,were selected randomly and divided equally into 5 groups.Pigs in group 1,2,3 and 4 were injected with 0.5,1,2 and 3 mL of swine pseudorabies inactivated vaccines (PRV JS-2012 gI/gE strains),respectively.Twenty eight days later,a booster vaccination was given at the same dosage.Group 5 was control group without vaccination.On 28 days post second immunization,all pigs were infected with 10s TCID50 JS-2012-F5 strain.The results showed that PRV gB antibodies were positive in group 2-4 at 14 dpi(days post infection),while the gB antibodies became positive in group 1 at 21 dpi.After infected with JS-2012-F5 strain,one piglet in group 1 showed clinical symptoms of pseudorabies disease,and the others only showed transient fever without any other abnormal clinical manifestations,which meant the protection rate was 80％.In group 2-4,piglets showed transient fever without any other clinical symptoms,so the protection rate was 100％.The temperature of the piglets in group 5 rose quickly above 41℃ after infected with JS-201-F5 for 48 hours,and the piglets showed obvious depression,anorexia and neurological symptoms,which indicated the incidence rate in control group was 100％.According to the above results,the minimal effective dose of vaccines against Pseudorabies virus was 1 mL per piglets.关键词
伪狂犬病毒/灭活疫苗/最小免疫剂量Key words
Pseudorabies virus/inactivated vaccine/minimum immunization dose分类
农业科技引用本文复制引用
童武,王涛,郑旭晨,单同领,童光志,李国新,郑浩,刘飞,梁超,田青,李林,曹艳云,武吉强..猪伪狂犬病基因缺失灭活疫苗(JS-2012-△gI/gE株)最小免疫剂量测定[J].中国动物传染病学报,2017,25(1):26-31,6.基金项目
上海市自然科学基金项目(14ZR1448900) （14ZR1448900）
上海市科技兴农重点攻关项目(沪农科攻字(2015)第1-7号) （沪农科攻字(2015）
国家生猪现代产业技术体系项目(CARS-36) （CARS-36）
公益性行业(农业)科研专项(201203039) （农业）
