中国动物传染病学报2017，Vol.25Issue(1)：45-49,5.
副猪嗜血杆菌ompP2基因的克隆鉴定及序列分析
IDENTIFICATION AND SEQUENCE ANALYSIS ON THE OMPP2 GENE OF HAEMOPHILUS PARASUIS
摘要
Abstract
We used PCR to determine the ompP2 gene of 15 H.parasuis local isolates and 2 reference strains.Then we sequenced the ompP2 gene of H.parasuis.All ompP2 gene sequence from H.parasuis,were analyzed for phylogenetic relationship.An approximately 1.1 kb fragment was obtained from the genomic DNA of 17 H.parasuis strains.Sequence analysis showed that the size of the ompP2 gene was difference,and had two conserved sequences of 34 ～ 39 amino acids in strains H37,H38 and H49.Beta folding prediction showed which had more than one ring structure and relatively far distance in strains H37,H38 and H49 than other strains.These results laid the foundation for further verification of the function and related research ofompP2 protein.关键词
副猪嗜血杆菌/ompP2基因/序列分析/进化树Key words
Haemophilus parasuis/ompP2 gene/sequence analysis/phylogenetic tree分类
农业科技引用本文复制引用
贾爱卿,王贵平,樊惠英,廖明..副猪嗜血杆菌ompP2基因的克隆鉴定及序列分析[J].中国动物传染病学报,2017,25(1):45-49,5.基金项目
农业部公益性行业科研专项经费项目(201303034) （201303034）
