中国体外循环杂志2017，Vol.15Issue(1)：5-8,22,5.DOI:10.13498/j.cnki.chin.j.ecc.2017.01.02
自体冷血停搏液对未成熟心肌保护作用与磷酸化蛋白激酶的相关性研究
Research on correlation between the protection effect of cold autologous blood cardioplegia solution to immature myocardium and the P-AKT
摘要
Abstract
Objective To explore the correlation between the protection effect of cold autologous blood cardioplegia solution to immature myocardium and the phosphorylated protein kinase B (P-AKT).Methods 82 infants with cyanotic congenital heart disease undergoing cardiac surgery were randomly divided into cold non-autologous blood cardioplegic solution (A) group (n=33) and cold autologous blood cardioplegic solution group (B) group (n=49).The right auricle tissue was taken before the aortic cross-clamping and 30 minute after the aortic cross-clamping to detect myocardial cell apoptosis and calculate the apoptosis index (AI) with TUNEL method,and to detect the phosphorylated AKT (P-AKT) with the immunohistochemical method.The auto-beating time and rate during operation,the left ventricular myocardial performance index (Tei Index),the cardiac Index (CI),left ventricular ejection fraction (LVEF),positive inotropic drug administration 2 hs postoperatively,and the incidence rate of cardiac arrhythmia 24 hs postoperatively of all patients were recorded.Results There was no significant difference on the levels of P-AKT and AI before the aortic cross-clamping between 2 groups (P>0.05),while all significantly elevated 30 minutes after the aortic cross-clamping (p<0.05).There were significant differences on the auto-beating time,left ventricular Tei index,CI,LVEF and positive inotropic drug administration 2 hs postoperatively,the incidence rate of cardiac arrhythmia 24 hs postoperatively between two groups (P<0.05).The level of P-AKT in myocardial tissue was negatively correlated with AI,auto-beating time and Tei index (P<0.05),positively correlated with the LVEF and CI (P<0.05).Conclusion Compared with cold non-autologous blood cardioplegic solution,cold autologous blood cardioplegic solution can promote myocardial p-AKT protein expression,reduce myocardial cell apoptosis,and has better protective effects for immature myocardium.关键词
自体冷血停搏液/婴儿/未成熟心肌/先天性心脏病/蛋白激酶B/AKTKey words
Cold autologous blood cardioplegic solution/Infant/Congenital heart disease/Protein kinase B/AKT引用本文复制引用
马超,张青,沈定荣,王元祥,彭乐,孟保英,提运幸,丁以群..自体冷血停搏液对未成熟心肌保护作用与磷酸化蛋白激酶的相关性研究[J].中国体外循环杂志,2017,15(1):5-8,22,5.基金项目
深圳市科技计划项目(201303069) （201303069）
