中国畜牧兽医2017，Vol.44Issue(2)：319-326,8.DOI:10.16431/j.cnki.1671-7236.2017.02.003
山羊痘病毒TK基因功能缺失对其增殖的影响
Effect on Proliferation of Goat Pox Virus of TK Gene Deficiency
摘要
Abstract
In this study,the proliferation of goat pox virus (GPV) was researched using the recombinant GPV of TK gene insertion inactivation.Different length DNA fragments X1,X2,X3 and X4 were inserted into the transfer plasmid pTKfpgigp to constructed the recombinant transfer plasmids and these plasmids were transfected into lamb testis cells infected GPV.Recombinant GPVs of TK gene inactivation by inserting foreign DNA fragment of 2 800,4 000,5 200 and 7 700 bp,respectively,were generated via homologous recombination.Four recombinant GPVs (rGPV/tk-) were obtained by selective culture and the virus titer were mensurated by 50％ tissue culture infective dose.The results showed that the rGPV/tk virus titers were decreased obviosuly which was comparable with the wild type GPV AV41.Furthermore,the virus titer of the recombinant virus was decreased more obviously with the extension of the foreign DNA fragment.The result indicated that the proliferation of the GPV was depressed when GPV TK gene was deficient.关键词
山羊痘病毒/TK基因/同源重组/病毒滴度Key words
goat pox virus/TK gene/homologous recombination/virus titer分类
农业科技引用本文复制引用
李继东,才学鹏..山羊痘病毒TK基因功能缺失对其增殖的影响[J].中国畜牧兽医,2017,44(2):319-326,8.基金项目
宁夏自然科学基金(NZ15050) （NZ15050）
