中国畜牧兽医2017，Vol.44Issue(2)：327-335,9.DOI:10.16431/j.cnki.1671-7236.2017.02.004
广东新型鸭呼肠孤病毒σB蛋白基因克隆及序列分析
Cloning and Sequence Analysis of σB Protein Genes of New-type Duck Reovirus Isolated from Guangdong
摘要
Abstract
In order to study the genetic variation and evolution of new-type duck reovirus (NDRV) in Guangdong province,some samples (liver and spleen) were collected from different sick ducks in Guangdong province.Eight strains were initially isolated and identified.σB protein genes were amplified,cloned and sequenced by RT-PCR method,and σB protein characteristics of amino acid,antigen and genetic analysis of system evolution were compared with other strains.The results showed that the isolated viruses were the NDRV.Comparison of nucleotide sequences showed the NDRV isolated from duck in Guangdong province were more closely related to DRV reported in other parts of the country,the homology reached 96.6％ to 99.5％,the NDRV isolated from duck in Guangdong province were not closely related with ARV and MDRV,the homology was 64.6％ to 66.5％ and 66.2％ to 67.1％,respectively.Homology comparison of nucleotide between 8 strains NDRV were 97.7％ to 99.7％.Compared with other strains,NDRV phosphorylation sites were less than ARV and MDRV reference strains,and the most area of the antigen index were high,similar to MDRV.Genetic evolution analysis results showed that NDRV and other domestic DRV were in the evolution of the same branch,but ARV and MDRV were in different branches.The result showed σB protein gene sequences of the NDRV were highly conserved,and NDRV isolated from Guangdong province had no significant regional differences with other regions.关键词
新型呼肠孤病毒/σB蛋白/克隆/序列分析Key words
new-type duck reovirus/σB protein/cloning/sequence analysis分类
农业科技引用本文复制引用
黄雯晶,梁国智,梅敏敏,李晓文,黄淑坚..广东新型鸭呼肠孤病毒σB蛋白基因克隆及序列分析[J].中国畜牧兽医,2017,44(2):327-335,9.基金项目
广东省家禽重大传染病控制技术研究(2012A020100001) （2012A020100001）
