中国畜牧兽医2017，Vol.44Issue(2)：336-343,8.DOI:10.16431/j.cnki.1671-7236.2017.02.005
珠江上游地区蚊虫乙型脑炎病毒核酸检测及PrM和E基因序列分析
Nucleotide Detection of Mosquito Japanese Encephalitis Virus and Sequence Analysis of PrM and E Genes in Upper Pearl River Region
摘要
Abstract
This experiment was conducted to understand mosquito species,distribution of the main vector of Japanese encephalitis virus (JEV),JEV genotype and molecular characteristics in upper Pearl River region.We collected mosquito and Culicoides specimen in Shizong county,upper Pearl River region in July 2013,and these specimens were classified and identified.Meanwhile,JEV nucleotide were detected using PrM and E genes specific primers in 162 pools of mosquito and Culicoides specimen,and the positive specimens were sequencd and analyzed by bioinformatics software.Total 3 705 mosquitoes were collected from 4 collection spots,and the mosquitoes belong to 7 species in 4 generas:Culex,Anopheles,Aedes and Armigeres.In cattle pens,Anopheles Sinensis and Armigeres subalbatus were the dominant mosquito species.In sheep pens,Anopheles Sinensis and Armigeres subalbatus were the dominant mosquito species.And in pig pens,Culex tritaeniorhynchus,Anopheles Sinensis and Armigeres subalbatus were the dominant mosquito species.4 of 162 pools mosquito and Culicoides were positive detecting by using PrM and E genes specific primers.Sequence analysis of JEV PrM and E genes results showed that 4 strains virus from Culex tritaeniorhynchus samples belonged to genotype Ⅰ JEV,and existing 15 amino acid difference sites between vaccine strain SA14-14-2 in the E gene protein,and 8 important sites which affected virus virulence did not mutant.These results suggested that there were many mosquito species in upper Pearl River region,and Culex tritaeniorhynchus,Anopheles Sinensis were dominant mosquito species in this region,and Culex tritaeniorhynchus was the main media of JEV in local region,genotype Ⅰ JEV was popular in local media,and virulence of the pandemic strain did not reduce.关键词
乙型脑炎病毒/蚊虫分类/库蠓/PrM基因/E基因Key words
Japanese encephalitis virus/classification of mosquito/Culicoides/PrM gene/E gene分类
医药卫生引用本文复制引用
吕顺燕,王静林,李楠,胡骑,何于雯,赵德宏,陈红宇,李华春..珠江上游地区蚊虫乙型脑炎病毒核酸检测及PrM和E基因序列分析[J].中国畜牧兽医,2017,44(2):336-343,8.基金项目
国家自然科学基金(31460669) （31460669）
国家公益性(农业)行业专项(201303035) （农业）
