中国畜牧兽医2017，Vol.44Issue(2)：357-364,8.DOI:10.16431/j.cnki.1671-7236.2017.02.008
牛GUCY1A3和SFXN1基因的克隆及生物信息学分析
Cloning and Bioinformatics Analysis of GUCY1A3 and SFXN1 Genes in Cattle
摘要
Abstract
In this study,the human GUCY1A3 and SFXN1 genes sequences in GenBank were chosen as probes to BLAST and got mRNA,and expressed sequence tags (ESTs) of bovine GUCY1A3 and SFXN1 genes.The bovine GUCY1A3 and SFXN1 genes were amplified by sequencing assembling of ESTs and cDNA sequences using RT-PCR.Then,we analyzed encoding proteins of bovine GUCY1A3 and SFXN1 genes with bioinformatics methods.Sequence analysis showed that the bovine GUCY1A3 gene CDS sequence was 2 076 bp,which encoded 691 amino acids,and the bovine SFXN1 gene contained a CDS region of 969 bp,which encoded 322 amino acids.The GUCY1A3 protein contained a CYCc domain,and the phosphorylation sites located in threonine,serine and tyrosine residue.The subcellular localization of GUCY1A3 protein was in the cytoplasm and it did not belong to the secreted protein.The secondary structure of GUCY1A3 protein was mainly composed of alpha helix.The SFXN1 protein contained three transmembrane helical regions and one low complexity sequence,and the phosphorylation sites located in serine,tyrosine and threonine residue.The subcellular localization of SFXN1 protein was in the endoplasmic reticulum and membrane,and it belonged to the transmembrane protein.The secondary structure of SFXN1 was mainly composed of alpha helix.The results laid the foundation for further studies of expression regulation mechanism and function of GUCY1A3 and SFXN1 genes in cattle.关键词
牛/GUCY1A3基因/SFXN1基因/电子克隆/生物信息学分析Key words
cattle/GUCY1A3 gene/SFXN1 gene/cloning/bioinformatics analysis分类
生物科学引用本文复制引用
靳聪飞,梁婷玉,刘新峰,郭宏..牛GUCY1A3和SFXN1基因的克隆及生物信息学分析[J].中国畜牧兽医,2017,44(2):357-364,8.基金项目
高产转基因肉牛新品种培育(2014ZX08007-002) （2014ZX08007-002）
