中国畜牧兽医2017，Vol.44Issue(2)：365-370,6.DOI:10.16431/j.cnki.1671-7236.2017.02.009
猪巨细胞病毒福建株DPOL基因的克隆及序列分析
Cloning and Sequencing Analysis of DPOL Gene of Porcine Cytomegalovirus
摘要
Abstract
In order to know the DPOL gene characterization of porcine cytomegalovirus (PCMV) (PCMV-FJ01 strain),three pairs of specific primer were designed by Oligo 7.0 software based on comparison the characterization of DPOL gene retrieved from GenBank.The target DPOL gene fragments were amplified using PCR methods with the strain PCMV-FJ01 genomic DNA.The target PCR fragments were cloned and sequenced.The assembly sequences were bioinformatics analysis.The DPOL gene of PCMV-FJ01 strain was 3 021 bp in length,coding 1 006 amino acids.The results showed that the homology of the nucleotide sequence and amino acid sequence were above 98.7％ and 99.1 ％,respectively.Phylogenetic analysis revealed that the PCMV was closer with genus Roseolovirus rather than genus Cytomegalovirus.The results suggested that PCMV should be new species under genus Roseolovirus base on the phylogenetic relationship.关键词
猪巨细胞病毒/DPOL基因/克隆/序列分析/病毒分类Key words
porcine cytomegalovirus/DPOL gene/clone/sequences analysis/virus classification分类
农业科技引用本文复制引用
陈如敬,陈铖,吴学敏,车勇良,严山,王晨燕,王隆柏,周伦江..猪巨细胞病毒福建株DPOL基因的克隆及序列分析[J].中国畜牧兽医,2017,44(2):365-370,6.基金项目
福建省属公益类科研院所基本科研专项(2014R1023-13、2014R1023-16) （2014R1023-13、2014R1023-16）
