中国畜牧兽医2017，Vol.44Issue(2)：384-390,7.DOI:10.16431/j.cnki.1671-7236.2017.02.012
恩拉霉素生物检测方法的改进
Improved Biotin Detection Method of Enduracidinmold
摘要
Abstract
The national standard pinella mildew element biological detection method was improved,using dry filter paper method instead of cylinder plate method,the potassium content of different samples of pinella mold were determined,and the extract composition,extraction time,the concentration of bacteria test and other factors influence on the test results of the established enramycin between concentration and the antibacterial circle diameter of linear equations was studied.The results showed that using acetone extraction liquid acetone (A ∶ 1 mol/mL HCl ∶ water =35∶12∶56,pH value was adjusted to 3.0 with 1 mol/mL HCl) leaching provided sample,extraction time of 3.5 h could fully immersed test of enramycin.Use of biological indicator bacteria CMCC (B) 63501 spore count of 3.4× 107 per mL of the measurement of living flat dish,the formation of the inhibitory circle edge rule was clear,good accuracy.Using dry filter paper method could effectively eliminate the extraction or dilute acidic fluid itself to detect strains inhibitory effect,and the utility model had the advantages of simple operation,good repeatability.It could be used for large quantities of samples of enramycin content determination.Enramycin standard solution at concentrations for 150～500 U/mL and its bacteriostatic circle diameter and valence have good linear regression.The resulting regression equation was Y=0.00944X+11.55344,the correlation coefficient was R=0.9962,the final effective range was 0.75～2.5 U,the measured results were more close to the true value.It could be used as an efficient enduracidin determination method,worthy of promotion and use.关键词
恩拉霉素/生物检测法/干燥滤纸片法/管碟法Key words
enduracidin/biological detection method/dry filter method/tube plate method分类
农业科技引用本文复制引用
许铭玉,宋淑婷,张莹,张会图..恩拉霉素生物检测方法的改进[J].中国畜牧兽医,2017,44(2):384-390,7.基金项目
Ficellomycin的生物合成及组合生物合成研究(81373309) （81373309）
