中国畜牧兽医2017，Vol.44Issue(2)：417-424,8.DOI:10.16431/j.cnki.1671-7236.2017.02.017
马骨髓间充质干细胞向软骨细胞的诱导分化
Study on Inducing Equine Bone Marrow Mesenchymal Stem Cells Differentiated into Chondrocytes
摘要
Abstract
This study was aimed to establish equine bone marrow mesenchymal stem cells(BM-SCs) line and induce it to differentiate into chondrocytes.The BMSCs were collected by cutting the bone and flushing the cutting surface by PBS,and then the bone marrow was washed with PBS,the collected cells were cultured after centrifugation.The cells were purified by passaging,the stem cell properties were tested before the induction.And the cells were also appraised by the expression of the special gene of chondrocytes as well as staining the differentiated cells with Alcian blue to insure the induction was effective.The obtained BMSCs expressed Sox2 and Nanog genes,which were the stem cell special genes,and also expressed CD44,CD90 and CD105 genes,but absent of CD34 and CD45 genes.The shapes of the 3rd passage BMSCs were changed after cultured in inducing medium for a few days.Furthermore,the cells were positive to Alcian blue staining,increased expression of the Col special gene of chondrocyte day by day as well.According to this study,the BMSC line was established,and the BMSCs were induced and differentiate into chondrocytes successfully.关键词
马/骨髓间充质干细胞/诱导分化/软骨细胞Key words
equine/bone marrow mesenchymal stem cells/induced differentiation/chondrocyte分类
生物科学引用本文复制引用
刘乐,王彦妮,庞玉娟,张婷婷,刘紫佳,朱梦,曹俊伟,张焱如,刘春霞..马骨髓间充质干细胞向软骨细胞的诱导分化[J].中国畜牧兽医,2017,44(2):417-424,8.基金项目
内蒙古自治区自然科学基金(2016MS0315) （2016MS0315）
内蒙古自治区高等学校科学研究项目(NJZY16066) （NJZY16066）
内蒙古农业大学生命科学学院师生协同科技创新项目(201501) （201501）
