中国畜牧兽医2017，Vol.44Issue(2)：554-560,7.DOI:10.16431/j.cnki.1671-7236.2017.02.035
鸭1型甲肝病毒亚型VP1蛋白单克隆抗体的研制及鉴定
Development and Identification of Monoclonal Antibodies against VP1 Protein of DHAV-1a
摘要
Abstract
To prepare the monoclonal antibodies (MAbs) against DHAV-1a,hybridomas were produced by fusing SP2/0 cells with spleen cells from mouse immunized with DHAV-1a pGEX-VP1 recombinant protein.Two hybridomas stablely secreting MAbs against VP1 protein were identified by indirect ELISA detection with DHAV-1a as coating antigen.The ascetic fluids of MAbs were 1∶3.2 × 104 and 1 ∶ 2.0 × 106,respectively.The MAbs were IgG1 with κ chain.Western blotting analysis showed that the MAbs could recognize the recombinant VP1 protein and DHAV-1a.The neutralization tests showed that one MAb (5G3) had better neutralization activity.Therefore,the results showed that the ELISA titers and specialities of two MAbs were very good,with excellent stability.In addition,they all had cross interaction with DHAV-1 and DHAV-1a,one of which had good neutralization activity.关键词
鸭1型甲肝病毒亚型/单克隆抗体/生物学特性Key words
DHAV-1a/monoclonal antibody/biological characteristics分类
农业科技引用本文复制引用
傅秋玲,陈珍,陈翠腾,林建生,刘伟,黄瑜,傅光华,万春和,程龙飞,陈红梅,施少华,刘荣昌..鸭1型甲肝病毒亚型VP1蛋白单克隆抗体的研制及鉴定[J].中国畜牧兽医,2017,44(2):554-560,7.基金项目
国家自然科学基金(31472222) （31472222）
现代农业产业技术体系建设专项(CARS-43) （CARS-43）
福建省公益类科研院所专项(2014R1023-3、2015R1023-3和2016R1102) （2014R1023-3、2015R1023-3和2016R1102）
福建省农业科学院畜牧兽医研究所基金(MYQJ2015-6) （MYQJ2015-6）
福建省优良蛋鸭品种与设施化养殖创新产业化工程项目(2014-2016) （2014-2016）
